Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques |
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Authors: | M. van Duren R. Morpurgo J. Dolezel R. Afza |
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Affiliation: | (1) Plant Breeding Unit, FAO/IAEA Agriculture and Biotechnology Laboratory, A-2444 Seibersdorf, Austria;(2) De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, Sokolovska 6, 77200 Olomouc, Czech Republic |
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Abstract: | Summary Flow cytometry and stomata characteristics were used for screening ploidy levels in a large population of in vitro induced autopolyploids of the Musa acuminata breeding clone SH-3362. Culturing shoot tips in liquid medium stipplemented both with 5.0 mM colchicine for 48 hours or 30 M oryzalin (3,5-dinitro-N4,N-dipropylsulphate) for seven days, both in combination with 2% (v/v) DMSO, resulted in a high (23.1% and 29.1%) frequency of non-chimeric tetraploids in the fourth vegetative generation. Although mixoploidy persisted in subsequent cycles of vegetative propagation, tetraploids as identified by flow cytometry remained solid non-chimeric during two more cycles. These autotetraploids were propagated for field testing. A rough pre-selection of regenerated V4 plants based on their stomata characteristics resulted in a population in which only 56.2% of the plants were solid tetraploids. The somatic polyploidization system reported here can be utilised for banana breeding programmes.Abbreviations FCM Flow Cytometry |
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Keywords: | banana breeding chimerism colchicine flow cytometry mixoploidy Musa oryzalin tetraploids |
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