Evaluation of the API 20E and Microbact 24E systems for the identification of Pseudomonas pseudomallei |
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Authors: | A D Thomas |
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Institution: | Oonoonba Veterinary Laboratory, Queensland Department of Primary Industries, P.O. Box 1085, Townsville, Queensland 4810 Australia |
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Abstract: | One-hundred isolates of Pseudomonas pseudomallei were used to evaluate the API 20E and Microbact 24E rapid identification systems. The API 20E system identified 50% of the isolates using the revised 1979 Manual only, and 63% when referral was made to the computer centre. A higher identification rate (69 and 87%, respectively) was achieved with a longer incubation period of 96 h. The Microbact 24E system identified 84% of the isolates as P. pseudomallei using the revised 1983 Manual, and 100% when referral was made to the computer centre. The Microbact 24E system would appear to be a reliable system for the identification of P. pseudomallei. |
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