苦豆子种质资源遗传多样性的RAPD分析

为深入研究并充分利用野生苦豆子（Sophora alopecuroides）资源，本研究利用RAPD技术对22个苦豆子居群进行研究，从DNA水平探讨苦豆子种质资源的遗传多样性。结果表明，筛选得到的31条RAPD随机引物共检测出314条扩增DNA片段，其中多态性条带285条，多态性比率为90.76%。Nei’s遗传多样性指数（H）为0.301 5，Shannon信息指数（I）为0.453 1，遗传距离变化范围为0.114～0.709，所研究的苦豆子居群间具有丰富的遗传多样性。UPGMA聚类分析和主成分分析(PCA)结果显示，22个苦豆子居群聚为6类，不同居群间出现了一定的遗传分化。

Genetic diversity of Sophora alopecuroides germplasm resources based on RAPD analysis

Genetic diversity of Sophora alopecuroides germplasm resources was analyzed at DNA level by Random Amplified Polymorphic DNA (RAPD). A total of 314 DNA bands from 22 populations in different habitats were amplified with 31 reliable RAPD primers, of which 285 DNA bands (90.76%) were polymorphic. The Nei’s gene diversity (H) and Shannon information index (I) were 0.301 5 and 0.453 1, respectively. The genetic distances ranged from 0.114-0.709. The results of RAPD unweighted pair group method with arithmetic means (UPGMA) cluster and the principal component analysis (PCA) demonstrated that 22 S.alopecuroides populations were clustered into 6 groups, which means that the genetic differentiations exist among populations.