亚临界水法提取红三叶中的总异黄酮

应用亚临界水法对红三叶(Trifolium pratense)总异黄酮的提取工艺进行优化，选择提取温度、液料比、提取时间、提取压力等因素分别进行单因素和正交试验。结果表明，提取温度为120 ℃，提取时间为15 min，液料比为40 mL·g-1，压力为1.4 MPa条件下，异黄酮提取效果最佳，此时红三叶总异黄酮得率为33.61 mg·g-1。

Optimal method to extract total isoflavones from red clover by sub-critical water

The extraction process of extracting total isoflavones from red clove was optimized by using subcritical water method in this study. The extraction temperature, ratio of liquid to material, extraction time and extraction pressure were tested by a single factor anlysis and orthogonal experiment design. This study showed that the optimal conditions were that the extraction temperature was 120 ℃, extraction time was 15 min, ratio of liquid to material was 40 mL·g-1 and extraction pressure was 1.4 MPa.The extracting rate of the total isoflavones from the red clove was 33.61 mg·g-1.