| 大豆凝集素、脲酶和胰蛋白酶抑制剂的分离纯化 |
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| 引用本文： | 张洪渊,刘克武,杨守忠,罗胜清. 大豆凝集素、脲酶和胰蛋白酶抑制剂的分离纯化[J]. 中国油料作物学报, 1991, 0(1) |
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| 作者姓名： | 张洪渊 刘克武 杨守忠 罗胜清 |
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| 作者单位： | 四川大学生物系(张洪渊，刘克武，杨守忠)，四川大学生物系(罗胜清) |
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| 摘 要： | 采用硫酸铵盐析、羟基磷灰石柱层析以及Sephadex凝胶过滤等方法,同时从大豆中分离纯化大豆凝集素、脲酶和胰蛋白酶抑制剂。三者的蛋白质回收率分别为0.39％、0.23％和0.13％。本方法与DEAE—Sephadex A_(50)柱层析及酸化—Sepharose 4 B、胰蛋白酶—Sepharose 4 B、卵类粘蛋白—Sepharose 4 B亲和层析分离纯化方法作了比较。本法简便易行,效果较好。
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| 关 键 词： | 大豆 凝集素 胰蛋白酶抑制剂 脲酶 |
PURIFICATION OF LECTIN,UREASE AND TRYPSIN INHIBITOR FROM SOYBEAN |
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| Zhang Hongyuan Liu Kewu Yang Shouzhong Luo Shengqing. PURIFICATION OF LECTIN,UREASE AND TRYPSIN INHIBITOR FROM SOYBEAN[J]. Chinese Journal of Oil Crop Sciences, 1991, 0(1) |
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| Authors: | Zhang Hongyuan Liu Kewu Yang Shouzhong Luo Shengqing |
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| Abstract: | A simplified method of isolation and purification of lectin,urease and trypsin inhibitor from soybean was,applied to extracted by(NH4)2SO4 fractionation,hydroxyapatite chrotnatography aad gel filtration chromatog-caphy on Sephadex G-200.The protein yield of lectin,urease and trypsin inhibitor were 0.39%,0.23% and 0.13%,respectively.The method had been copmared with some other methods containing DEAF-Sephadex A50 chromatography,affinity chromatography on acidic Sepharose 4B,trypsin-Sepharose 4B and ovomucoid-Sepha,rose 4B column,respectivly.This is a good and simple method. |
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| Keywords: | Soybean Lectih Urease Trypsin inhiditor |
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