甜杨葡萄糖-6-磷酸脱氢酶基因的电子克隆及其功能预测

［目的］对甜杨葡萄糖-6-磷酸脱氢酶（G6PDH）进行克隆及功能预测。［方法］采用电子克隆和RT-PCR相结合的方法首次从甜杨中分离了G6PDH基因，通过Blast和其他生物信息学软件进行功能预测。［结果］甜杨G6PDH基因的cDNA长1697bp，可编码510个氨基酸，cDNA序列及其推导的氨基酸序列均与其他植物G6PDH存在着较高的同源性，说明所获得的cDNA是甜杨G6PDH基因（PsG6PDH，AY445917）。同时，运用PCR扩增技术获得了甜杨G6PDH基因组序列，测序结果表明，基因组DNA长度为5 040bp，含有15个外显子和14个内含子。Southern杂交结果表明，G6PDH基因在甜杨的基因组中可能是单拷贝或者低拷贝。通过网络服务器平台进行PsG6DH的功能预测，结果显示，PsG6PDH为G6PDH的成员之一，含有NADP结合区和G6P结合区2个保守功能域，具有多个磷酸化位点和跨膜区域，但不含有信号肽，表明PsG6DH可能作为膜受体而起作用。另外，G6PDH的电子表达谱分析结果发现，G6PDH在多种组织和不同发育过程均表达，并涉及各种逆境胁迫应答反应，这也说明了G6PDH在植物生长反育中的重要地位。［结论］可为下一步研究PsG6PDH基因的分子功能提供参考。

In Silico Cloning and Function Prediction of a Glucose-6-phosphate Dehydrogenase Gene from Populus suaveolens

[Objection]In silico cloning and function prediction of glucose-6-phosphate dehydrogenase gene(G6PDH) was researched in the paper.[Method] The G6PDH gene was cloned from P.suaveolens by electronic PCR and RT-PCR,and function prediction was carried out by Blast and other bioinformatics softs.[Result] The full length PsG6PDH was 1 697 bp and encoded 510 amino acids.cDNA and amino acid sequences showed high identity with other plant G6PDH genes.P.suaveolens(fragrant poplar) G6PDH genomic DNA was isolated by PC...