| 裸花紫珠组培快繁技术研究 |
| |
| 引用本文： | 黄东梅,李敬阳,林妃,许奕,李羽佳. 裸花紫珠组培快繁技术研究[J]. 农学学报, 2014, 4(12): 63-66. DOI: 10.11923/j.issn.2095-4050.2014-xb0577 |
| |
| 作者姓名： | 黄东梅 李敬阳 林妃 许奕 李羽佳 |
| |
| 作者单位： | 中国热带农业科学院海口实验站,中国热带农业科学院海口实验站,中国热带农业科学院海口实验站,中国热带农业科学院热带生物技术研究所,中国热带农业科学院海口实验站 |
| |
| 基金项目： | 海南省产学研一体化专项资金(CXY30130035)。 |
| |
| 摘 要： | 以裸花紫珠无菌苗幼嫩茎段为外植体，研究不同激素配比下芽诱导、增殖和生根情况，获得一套裸花紫珠快速繁殖的方法。结果表明：裸花紫珠幼嫩茎段诱导不定芽的最适培养基为MS+ 6-BA 2.0 mg/L+ NAA 0.1~0.3 mg/L；不定芽在添加6-BA和NAA的各处理上均可增殖，在MS+ 6-BA 1.0 mg/L+ NAA 0.3 mg/L上增殖系数最高、芽苗健壮；最佳生根诱导培养基为1/2MS+ NAA 0.05 mg/L+ IBA 0.5 mg/L。此方法可为裸花紫珠快繁提供技术支持。
|
| 关 键 词： | 过碳酸钠 过碳酸钠 次氯酸钠 升汞 烟草种子 表面灭菌 萌发 植株生长 |
| 收稿时间： | 2014-06-10 |
| 修稿时间： | 2014-06-10 |
Study on Fast Propagation via Tissue Culture for Callicarpa nudiflora |
| |
| Huang Dongmei,Lin Fei,Xu Yi,Li Yujia,Li Yanxia,Ma Weihong,Li Jingyang. Study on Fast Propagation via Tissue Culture for Callicarpa nudiflora[J]. Journal of Agriculture, 2014, 4(12): 63-66. DOI: 10.11923/j.issn.2095-4050.2014-xb0577 |
| |
| Authors: | Huang Dongmei Lin Fei Xu Yi Li Yujia Li Yanxia Ma Weihong Li Jingyang |
| |
| Affiliation: | Huang Dongmei, Lin Fei, Xu Yi, Li Yujia, Li Yanxia, Ma Weihong, Li Jingyang (1Hainan Key Laboratory of Banana Genetic Improvement, Haikou Experimental Station/ Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, Hainan, China; 2Seedlings Cultivation Center, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, Hainan, China) |
| |
| Abstract: | Using youngest stem of Callicarpa nudiflora as explant, the effects of different kinds and concentrations of plant grows regulator on shoot induction, proliferation and rooting were studied in order to get a unit of technology on fast propagation. Result showed that adventitious buds could be fast induced on medium MS＋6-BA 2.0 mg/L＋NAA 0.1-0.3 mg/L, and the optimum proliferation medium was MS＋6-BA 1.0 mg/L＋NAA 0.3 mg/L＋GA3 0.5 mg/L, on which could get the highest proliferation rate and strongest shoots. Root could be induced best on medium 1/2MS＋NAA 0.05 mg/L＋IBA 0.5 mg/L. This method could provide technical support for large-scale propagation of Callicarpanudiflora. |
| |
| Keywords: | Callicarpa nudiflora Hook. Et Arn Tissue Culture Fast Propagation |
| 本文献已被 维普 等数据库收录！ |
| 点击此处可从《农学学报》浏览原始摘要信息 |
|
点击此处可从《农学学报》下载免费的PDF全文 |
|
