| 3种桑蟥基因组DNA提取方法比较研究 |
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| 引用本文： | 王钰婷,汪泰初,李瑞雪,王伟.3种桑蟥基因组DNA提取方法比较研究[J].农学学报,2014,4(12):95-98. |
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| 作者姓名： | 王钰婷 汪泰初 李瑞雪 王伟 |
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| 作者单位： | 安徽省农业科学院蚕桑研究所,安徽省农业科学院蚕桑研究所,安徽省农业科学院蚕桑研究所,安徽省农业科学院蚕桑研究所 |
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| 基金项目： | 安徽省农科院院长青年创新基金(13B0632)；国家蚕桑产业技术体系合肥综合试验站(CARS-22-SYZ09)；安徽省农科院创新团队项目(11C0610)。 |
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| 摘 要： | 为了探寻一种高效、稳定、廉价的桑蟥基因组DNA提取方法，通过采用CTAB法、蛋白酶K法和盐析法3种方法研究桑蟥基因组DNA的提取。对提取的DNA进行分光光度值的测定以及COI基因的扩增鉴定。结果表明：3种方法均可以提取基因组DNA，蛋白酶K法和CTAB法DNA获得率优于饱和NaCl法提取的DNA，3种方法提取的DNA都可用于PCR扩增实验。因此，在实际工作中可根据实验目的、条件选取最适合的方法。
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| 关 键 词： | 稻螟赤眼蜂 稻螟赤眼蜂 小菜蛾 甜菜夜蛾 生命表 |
| 收稿时间： | 2014/7/10 0:00:00 |
| 修稿时间： | 2014/7/10 0:00:00 |
Comparison of Three Methods for Genomic DNA Extraction from the Rondotia menciana Moore |
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| Wang Yuting,Wang Taichu,Li Ruixue,Wang Wei.Comparison of Three Methods for Genomic DNA Extraction from the Rondotia menciana Moore[J].Journal of Agriculture,2014,4(12):95-98. |
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| Authors: | Wang Yuting Wang Taichu Li Ruixue Wang Wei |
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| Institution: | (The Sericultural Research Institute, Anhui Academy of Agricultural Science, Hefei 230061, Anhui, China) |
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| Abstract: | To explore an efficient, stable, low-cost DNA extraction method from Rondotia menciana Moore, genomic DNA was extracted using CTAB, protein enzyme K and salting-out method, respectively. The extracted genomic DNA samples of three methods were tested using photometric analysis and COI gene amplification. The results showed that, all the three methods can be used for the total DNA extraction, which are suitable for the PCR amplification, but genome DNA extracted by CTAB method and protein enzyme K method were higher in purity and production, degraded slightly. So we should select the proper method according to the objective and condition in the study. |
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| Keywords: | Rondotia menciana Moore DNA Extraction CTAB Method Protein Enzyme K Method Salting-out Method |
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