Volatile metabolite profiling to detect and discriminate stem-end rot and anthracnose diseases of mango fruits |
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Authors: | M. Moalemiyan A. Vikram A. C. Kushalappa V. Yaylayan |
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Affiliation: | Plant Science Department;and;Food Science and Agric-Chemistry Department, McGill University, Ste-Anne-de-Bellevue, Quebec, H9X 3V9, Canada |
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Abstract: | The volatile metabolites from the headspace gas of containerised mango ( Mangifera indica ) cv. Tommy Atkins fruits, surface wounded and inoculated with the two fungal anamorphic pathogens Colletotrichum gloeosporioides and Lasiodiplodia theobromae , or non-inoculated (controls), were profiled using a portable gas chromatograph/mass spectrometer to discriminate diseases of mango. Thirty-four compounds were detected relatively consistently among replicates. Several of these were disease/inoculation-discriminatory and were classified into three groups: (i) compounds unique to only one treatment; (ii) compounds common to two or more treatments, but not to all; and (iii) compounds common to all treatments, but varying in their abundance. Two compounds, 1-pentanol and ethyl boronate, were detected in L. theobromae- inoculated mangoes alone, while thujol was observed only in C. gloeosporioides- inoculated mangoes. Discriminant analysis models based on the abundance of significant mass ions and consistent compounds correctly classified diseases/inoculations in up to 100% of cases. The disease-discriminatory compounds and discriminant analysis models developed here have the potential to be used in the early detection of postharvest diseases of mango fruits after validation under commercial conditions. |
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Keywords: | Colletotrichum gloeosporioides discriminant analysis disease detection gas chromatography/mass spectrometry Lasiodiplodia theobromae postharvest disease |
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