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| 甲烷氧化混合菌的高密度培养和PHB合成 | |
| 引用本文： | 张莹莹,董静,辛嘉英,姜丹,张颖鑫,夏春谷. 甲烷氧化混合菌的高密度培养和PHB合成[J]. 农产品加工．学刊, 2011, 0(11): 7-10. DOI: 10.3969/j.issn.1671-9646(X).2011.11.002 |
| 作者姓名： | 张莹莹 董静 辛嘉英 姜丹 张颖鑫 夏春谷 |
| 作者单位： | 1. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨,150076 2. 哈尔滨商业大学食品科学与工程省重点实验室,黑龙江哈尔滨150076;中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州730000 3. 中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,甘肃兰州,730000 |
| 基金项目： | 国家自然科学基金资助项目，黑龙江省教育厅重点项目，黑龙江省普通高等学校科技创新团队建设计划项目 |
| 摘 要： | 甲烷氧化菌混合菌群是一类能够以甲烷和甲醇等C1-化合物为碳源生长,并合成PHB的混合微生物。研究了甲烷氧化混合菌培养基中重要营养成分,包括NH4+,NO3-,HPO42-,H2PO4-,Fe3+,Mg2+浓度以及碳源对细胞生长、PHB积累的影响作用。以此为依据,建立适于混合菌高密度生长的第一阶段和高PHB含量的第二阶段的培养基组成。通过二阶段发酵培养,细胞干质量浓度达到1.67 g/L,PHB质量分数达到61.5%,产量为1.021 g/L,为PHB的工业化生产奠定了基础。 |
| 关 键 词： | 甲烷氧化混合菌 甲烷 甲醇 PHB |
High Cell Density Cultivation and PHB Biosynthesis of Methane-utilizing Mixed Culture |
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| Zhang Yingying,Dong Jing,Xin Jiaying,Jiang Dan,Zhang Yingxin,Xia Chungu. High Cell Density Cultivation and PHB Biosynthesis of Methane-utilizing Mixed Culture[J]. Nongchanpin Jlagong.Xuekan, 2011, 0(11): 7-10. DOI: 10.3969/j.issn.1671-9646(X).2011.11.002 | |
| Authors: | Zhang Yingying Dong Jing Xin Jiaying Jiang Dan Zhang Yingxin Xia Chungu |
| Affiliation: | 1.Key Laboratory for Food Science and Engineering,Harbin University of Commerce, Harbin,Heilongjiang 150076,China; 2.State Key Laboratory for Oxo Synthesis & Selective Oxidation,Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou,Gansu 730000,China) |
| Abstract: | Methanotrophs are aerobic microorganisms that can only utilize C1-such as methane and methanol as primary substrates to grow and synthesize PHB.In this paper,the effect of important nutrition in cultive medium,including ammonium, nitrate,phosphorus,iron or magnesium concentration and carbon sources on cells growth and PHB accumulation are studied. Based on the experiment date,the two stages medium composition are established:the first phase is suitable for high cell density cultivation and the second phase is for PHB accumulation.Through two stages fermentation,the highest density of cultivated cells is 1.67 g/L-1,and the PHB content of biomass is as high as 61.5%,the maximum yield reaches to 1.021 g/L. |
| Keywords: | methylococcus capsulatus HD6T methane methanol PHB |
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