Microsatellite analyses for evaluation of genetic diversity among Sicilian grapevine cultivars |
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Authors: | Francesco Carimi Francesco Mercati Loredana Abbate Francesco Sunseri |
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Institution: | 1.Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche,Palermo,Italy;2.Dipartimento di Biotecnologie per il Monitoraggio Agro-alimentare ed Ambientale,Università Mediterranea degli Studi di Reggio Calabria,Reggio Calabria,Italy |
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Abstract: | A total of 82 grapevine genotypes were sampled from several areas of the Italian region of Sicily where vineyards are widely
spread. The grapevines were characterized using six microsatellite markers (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62 and VrZAG79)
to evaluate genetic diversity. Thirty-seven of the 82 cultivars sampled had their names quoted in historical and literary
sources, while 45 cultivars from old vineyards did not have their names reported in ancient literature. According to their
genetic profiles at SSR loci, 70 different cultivars were found, while interesting cases of synonymies (Regina and Moscato
bianco, Alicante and Dolcetta or among different clones of Zibibbo and Catarratto) and cases of homonymy (Frappato and Nerello
Mascalese) were discovered. Several genetic parameters were calculated to assess the efficacy of the loci chosen in this work.
Pairwise genetic distances between all cultivars were calculated. A dendrogram representing the genetic similarities among
cultivars was depicted using the UPGMA method to investigate their relationships, explaining them from a historical point
of view. The cluster distribution of cultivars clearly does not reflect their current geographical distribution, suggesting
successive introductions of cultivars in Sicily from different areas of origin. |
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