利用RAPD标记鉴定大豆种质

本研究以５７个中国大豆祖先吕系及育成品种和１８个美国大豆祖先品系为ＤＮＡ样品来源，通过随机引物ＰＣＲ扩增基因组ＤＮＡ的多态性，探索利用ＲＡＰＤ标记鉴定和相关种质的可能性。研究结果表明，５０个１０摩尔随机引物共扩增可分辩产物２４６个，其中８２．４％的随机引物可产生多态性产物，所扩增产物的５４．４％至少在两个基因毒草境存在差异。上ＰＣＲ扩增产物分别以１和０记录存在与否。扩增产物间的成对比较可产生非相似

Evaluation of Soybean Germplasm with Random Amplification Polymorphic DNA(RAPD)Markers

Fifty-seven Chinese ancestral lines and their derived cultivars and eighteen US ancestral lines were used to evaluate genetic diversities by RAPD markers between or within Chinese and US lines. Fifty 10-mer random primers were used for PCR reactions. The total of 246 distinguishable PCR products were detected by agarose gel electrophoresis among 75 accessions, 82. 3% of random primers were polymorphic and 54. 4% of total produts were different at least between two lines, which can be used as RAPD markers to identify soybean germplasm resources. Thirty-two soybean accessions had their special PCR banding patterns. Each of those amplified bands was taken as one of the variant which was record as 1 for present and 0 for absent. Comparison among all possible a pair of data were calculated to produce non-similarity matrix and to build up a pedigree tree. The cluster analysis indicated that not only Chinese ancestral lines were different from US ancestral lines, but northern soybean accesstions were different from southern ones within two countries also. The obvious geographic differences provide possibilities to broaden genetic base of Chinese soybean cultivars by selecting germplasm from various regions within or between countries.