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桑黄菌丝固体培养基及培养条件研究
引用本文:刘红锦,张苏珍,蒋宁,陈忠立,孙道权.桑黄菌丝固体培养基及培养条件研究[J].江西农业学报,2012(11):123-124,128.
作者姓名:刘红锦  张苏珍  蒋宁  陈忠立  孙道权
作者单位:江苏省农业科学院农产品加工研究所;国家桑蚕茧丝产业工程技术研究中心;鑫缘茧丝绸集团股份有限公司
基金项目:江苏省科技支撑计划(BE-2010404);江苏省农业综合开发科技推广(2011kj-39)
摘    要:首先利用单因素试验对桑黄菌丝培养基基本成分进行确定,然后利用正交设计对其配比及生长条件加以优化,得出最佳配比及条件:葡萄糖20 g,蛋白胨2.2 g,磷酸二氢钾0.46 g,磷酸氢二钾1.0 g,硫酸镁0.5 g,琼脂20.0 g,水1000 mL,pH为7,培养温度为28℃。

关 键 词:桑黄  菌丝  培养条件

Study on Solid Medium and Culture Condition of Phellinus baumii
LIU Hong-jin,ZHANG Su-zhen,JIANG Ning,CHEN Zhong-li,SUN Dao-quan.Study on Solid Medium and Culture Condition of Phellinus baumii[J].Acta Agriculturae Jiangxi,2012(11):123-124,128.
Authors:LIU Hong-jin  ZHANG Su-zhen  JIANG Ning  CHEN Zhong-li  SUN Dao-quan
Institution:1.Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;2.National Mulberry Silk Industrial Engineering Technology Research Center,Nantong 226600,China;3.Xinyuan Cocoon Silk Group Company Limited,Nantong 226600,China)
Abstract:The medium components for Phellinus baumii mycelial growth were analyzed using the single factor experiment.Then their ratio and growth condition were optimized by orthogonal experimental analysis.The optimal components ratio and condition were as follows: 20 g glucose,2.2 g peptone,0.46 g monopotassium phosphate,1.0 g dipotassium hydrogen phosphate,0.5 g magnesium sulfate,20.0 g agar,1000 mL water,pH 7,and temperature was 28 ℃.
Keywords:Phellinus baumii  Mycelium  Culture conditions
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