| 粘虫(Mythimna separata Walker)幼虫连续石蜡切片制备之幼虫整体固定方法初探 |
| |
| 引用本文： | 王海舟,王小奇,吕 芳,崔 蕾,王 璐. 粘虫(Mythimna separata Walker)幼虫连续石蜡切片制备之幼虫整体固定方法初探[J]. 中国农学通报, 2006, 22(8): 435-435 |
| |
| 作者姓名： | 王海舟 王小奇 吕 芳 崔 蕾 王 璐 |
| |
| 作者单位： | 1. 沈阳农业大学植物保护学院,辽宁,沈阳,110161
2. 辽中县农业推广中心,辽中,110200 |
| |
| 摘 要： | 笔者首先从18种动物学制片常用的固定液中，选出9种较为适合鳞翅目幼虫固定的固定液，然后又通过进一步试验发现：30倍液量为固定液的最少用量；35℃为固定时的最适温度；5龄幼虫为固定的最佳龄期；4龄幼虫蜕皮后20min之内为固定的最佳时段
|
| 关 键 词： | 粘虫 幼虫 整体固定 |
| 收稿时间： | 2006-04-19 |
| 修稿时间： | 2006-04-192006-05-25 |
Preparation of Serial Paraffin Slices of Mythimna separata Walker: Preliminary Research on the Fixing Methods of the Whole Larval Body of Oriental Armyworm |
| |
| Wang Haizhou,Wang Xiaoqi,Lv Fang,Cui Lei,Wang Lu. Preparation of Serial Paraffin Slices of Mythimna separata Walker: Preliminary Research on the Fixing Methods of the Whole Larval Body of Oriental Armyworm[J]. Chinese Agricultural Science Bulletin, 2006, 22(8): 435-435 |
| |
| Authors: | Wang Haizhou Wang Xiaoqi Lv Fang Cui Lei Wang Lu |
| |
| Affiliation: | Plant Protection College of Shenyang Agricultural University, Shenyang 110161 |
| |
| Abstract: | 9 kinds of fixation fluids were firstly selected from the 18 kinds which were commonly used in biological section-making in zoology in the paper, which are comparatively suitable for the larvae of Lepidoptera. Then through further tests, it was found that: Liquid amount of 30 times to the larvae’s volume was the least consumption of the fixation fluids. 35℃ was the most suitable fixing temperature. The larvae in the fifth age were the best fixing ones. It was the regular best fixing period within 20 minutes after the larva casted off a skin in the fourth age. |
| |
| Keywords: | Mythimna separata Walker Larva Fixation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
|
点击此处可从《中国农学通报》下载全文 |
