熏蒸时间对牛冻精活力的影响

牛精液进行冷冻处理，利用不同初冻方法，对比精子活力效果。熏蒸时间分为6、8、10min3个试验组合，采用常规牛冻精稀释液配方。试验结果：熏蒸时间6、8、10min3个试验组精子活力分别为0．286土0．0353、0．351土0．0326和0．319±0．0307，其中试验2组最高，试验3组次之，试验1组最低。精子顶体完整率分别为37．83、46．36和42．62，其中试验2组高于试验1组，其它组间比较差异不明显。畸形率分别为29．62、24．02和26．58，试验2组和试验3组间差异不显著，两组与试验1组相比差异均显著。试验表明，在熏蒸距离为2cm时，熏蒸时间控制在8min，其精液解冻后精子活力符合牛输精要求的国家标准。

Effect of Steaming Time on Bull Frozen Semen Activity

The study investigated the semen activity of using different initial freezing method. Three experimental groups of 6, 8 and 10 min fumigation time and normal bovine frozen semen dilution formula were adopted. The results indicated that se- men activities were 0. 286±0. 0353,0. 351：J=0. 0326 and 0. 319±0. 0307,group 2 showed the highest,the acrosome integrity rates were 37.83,46.36 and 42.62, group 2 was higher than group 1,the deformity rates were 29.62,24.02 and 26.58,there was no significant difference among them. To sum up, under the condition of the proper steaming distance of 2 cm and the prop- er steaming time of 8 min to 10 min,thawing quality and semen activity is in accordance with the scope of bovine semen.