Existence of two serological subclusters of Plum pox virus, strain M |
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| Authors: | Arben Myrta Donato Boscia Oriana Potere Maria Kölber Maria Németh Biagio Di Terlizzi Mariano Cambra Vito Savino |
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| Institution: | (1) Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy;(2) Istituto Agronomico Mediterraneo, Valenzano, (BA), Italy;(3) Dipartimento di Protezione delle Piante e Microbiologia Applicata, Università and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Bari, Italy;(4) Plant Health and Soil Conservation Station, Budapest, Hungary;(5) Instituto Valenciano de Investigaciones Agrarias (IVIA) Moncada, Valencia, Spain |
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| Abstract: | A large-scale serological characterisation of Plum pox virus (PPV) isolates was carried out with 19 monoclonal antibodies (MAbs), including the universal MAb5B and the following strain-specific MAbs: AL (specific to PPV-M), 4DG5 (specific to PPV-D), TUV and AC (specific to PPV-C), and EA24 (specific to PPV-EA). The study involved 108 PPV isolates of different geographical origin (Albania, Bulgaria, Cyprus, Czech Republic, Egypt, France, Germany, Greece, Italy, Hungary, Moldova, Romania, Slovakia, Spain, Turkey and Yugoslavia) and hosts (almond, apricot, peach, plum and cherry). The inter- and intra-strain serological relationships of PPV isolates were evaluated by DASI-ELISA. High serological variability was detected, not only between strains, but also among isolates of the same strain. Computer-assisted analysis of serological data support the hypothesis of the existence of two distinct subclusters, denoted PPV-M1 and PPV-M2, which seem to prevail in Mediterranean and Eastern–Central European countries, respectively. |
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| Keywords: | PPV strain M serological subcluster MAbs ELISA |
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