| 罗尔斯通氏菌菌株H16新毒素蛋白Reu理化性质的研究 |
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| 引用本文： | 张一折,包振霞,刘国祥,刘永涛.罗尔斯通氏菌菌株H16新毒素蛋白Reu理化性质的研究[J].河南农业科学,2010(3). |
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| 作者姓名： | 张一折 包振霞 刘国祥 刘永涛 |
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| 作者单位： | 郑州大学,生物工程系,河南,郑州,450001 |
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| 摘 要： | 为深入研究罗尔斯通氏菌H16新毒素蛋白Reu的理化性质,以初孵菜青虫四龄幼虫为试虫,进行Reu胃毒毒力测定,计算其LD50,检测Reu的热稳定性及其对蛋白酶的敏感性,通过非变性梯度凝胶电泳测定Reu的分子量;SDS-PAGE电泳分析Reu的亚基组成;等电聚焦电泳测定Reu的等电点等。结果表明,Reu对菜青虫四龄幼虫毒力LD50为110.84μg/g;此毒素蛋白经80℃处理10min和蛋白酶K处理后,基本丧失了对菜青虫幼虫的毒杀作用;Reu的分子量为221.3kDa,由64.4kDa和57.5kDa2种亚基组成,等电点为7.08。
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| 关 键 词： | 细菌 罗尔斯通氏菌菌株H16 毒素蛋白Reu 理化性质 杀虫活性 |
Biophysical and Biochemical Properties of Ralstonia eutropha H16 Novel Toxin Protein Reu |
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| ZHANG Yi-zhe,BAO Zhen-xia,LIU Guo-xiang,LIU Yong-tao.Biophysical and Biochemical Properties of Ralstonia eutropha H16 Novel Toxin Protein Reu[J].Journal of Henan Agricultural Sciences,2010(3). |
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| Authors: | ZHANG Yi-zhe BAO Zhen-xia LIU Guo-xiang LIU Yong-tao |
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| Abstract: | To study biophysical and biochemical properties of Ralstonia eutropha H16 novel toxin protein Reu,four-age larvae of Pieris rapae was tested.Bioactivity mensuration revealed that Reu had a strong stomach cytotoxicity to Pieris rapae with the LD 50 reaching 110.84μg/g in death.The results obtained from non-denaturing gradient gel electrophoresis showed that Reu is 221.3kDa in molecular weight and comprised of two subunits,64.4kDa and 57.5kDa.Reu was separated into two different protein subunits during SDS-PAGE.Besides,Reu was sensitive to proteinase K treatment and toxicity of Reu was stable within 25-60℃.Its isoelectric point is 7.08. |
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| Keywords: | Bacterial Ralstonia eutropha H16 Toxin protein Reu Biophysical and biochemical property Insecticidal activity |
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