青山羊表皮生长因子基因表达与毛囊发育特性的研究

【目的】比较研究表皮生长因子（epidermal growth factor，EGF）基因的表达与青山羊皮肤毛囊发育之间的关系。【方法】用组织学技术和显微观测的方法，研究济宁青山羊1日龄、1-5月龄，共6个年龄段的皮肤毛囊的组织学特性，结合荧光定量PCR技术，研究其与EGF基因表达之间的关系，并用SAS9.0软件进行相关性分析。【结果】初级毛囊直径在出生后随日龄稍有增大，但差异不显著（P＞0.05），而次级毛囊直径在2月龄之前极显著增大（P＜0.01），达到发育高峰，之后保持相对稳定。EGF基因在出生后表达量极显著升高（P＜0.01），在2月龄达到最高，之后表达量极显著减少（P＜0.01）。【结论】初级毛囊在出生前就已全部发育形成，而次级毛囊在出生后可继续发育至2月龄。自出生至2月龄，EGF表达量与次级毛囊直径呈极显著正相关（P＜0.01）。

Studies on Expression Characteristics of EGF and Development Characteristics of Hair Follicle of Gray Goat

【Objective】 The relationship between the expression of epidermal growth factor and the development of hair follicle was studied.【Method】 The histology method,micro-observation approach and QRT-PCR were used to detect the relationship between the expression of EGF and the development of hair follicle of 1-day-old,1-month-old,2-month-old,3-month-old,4-month-old and 5-month-old Jining Gray goat.SAS9.0 was used to analyze the relationship between them.【Result】 The resuts indicated that the diameter of primary follicle increased,but not significantly(P>0.05).The diameter of secondary follicle increased very significantly(P<0.01).The diameter of secondary follicle reached peak of growth at 2-month-old.The expression of EGF increased after birth and achieved the highest at 2-month-old(P<0.01).It decreased extremely significant after 2-month-old(P<0.01).【Conclusion】 The primary follicle was completely developed before birth and the secondary follicle was developed after birth.The expression of EGF and the diameter of secondary follicle had a significant positive correlation before 2-month-old(P<0.01).The results indicated that EGF is significantly positive to the development of secondary follicle.