黄瓜黑斑病苗期抗病性鉴定方法及品种抗病性评价

黄瓜黑斑病近几年在全国各地普遍发生，已成为黄瓜生产上的重要病害。为适应黄瓜抗病育种的需要，对黄瓜黑斑病苗期人工接种抗病性鉴定技术进行了研究，筛选出适合于黄瓜黑斑病苗期抗病性鉴定的方法：于黄瓜幼苗1～2片真叶期时，采用分生孢子浓度为1×107～2×107个?mL-1的悬浮液喷雾接种，以叶面不形成水滴为度，接种温度22 ℃左右，黑暗保湿48 h|接种后幼苗置于培养箱中，白天28～30 ℃、夜间20 ℃，13 h光照/11 h黑暗，前48 h温度为22 ℃左右、相对湿度为100%|以后仅夜间保湿，5～15 d调查发病情况。利用该方法筛选出10份高抗材料，8份中抗材料，以及2个高抗品种D9和W3，4个抗病品种3-1、LW11、BA74和A8674。

Seedling Identification Method of Resistance to Cucumber Black Spot and Variety Resistance Evaluation

Black spot is one of the most severe diseases affecting cucumber(Cucumis sativus L.)production.It is an essential step to set up a methodology for artificial seedling identification of cucumber resistance to black spot in disease resistance breeding.Pycnoconidia spore suspension with concentration 1×107-2×107 per mL was used as identification method of resistance to cucumber black spot.The spore suspension was sprayed on to the seedlings with 1 or 2 true leaf,and incubated at 22 ℃ in growth chamber under humid conditions for 48 hours and keeping relative humidity 100%,then kept in the condition(day 28-30 ℃,night 20 ℃,13 hours light per day,11 hours in dark for 5-15 days)before being evaluated by scoring the disease-lesion on each plant leaf.The inoculation results showed that 10 accessions were high resistant and 8 accessions were mid-resistant to black spot.D9 and W3 were high resistant varieties and 3-1,LW11,BA74,A8674 were resistant varieties to black spot.