猪神经介素B和受体基因的克隆与组织分布

基于电子延伸序列,克隆并分析了猪神经介素B(NMB)和受体(NMBR)基因。猪NMBcDNA克隆片段长度为567 bp,开放阅读框架长度为366 bp,编码121个氨基酸。NMBR cDNA克隆片段长度为1 194 bp,开放阅读框架长度为1 173 bp,编码390个氨基酸。同源分析表明,猪NMB的核酸序列与牛、人、小鼠和大鼠的同源性分别为87.1%,85.2%,78.1%和76.6%,氨基酸序列的同源性分别为81%,74.4%,70.2%和70.1%;NMBR的核酸序列与牛、人、小鼠和大鼠的同源性分别为91%,89%,84.2%和83.6%,氨基酸序列的同源性分别为92.3%,90.3%,86.9%和86.4%。组织分布结果显示,猪NMB在多种组织均有分布,NMBR仅分布在大脑。克隆的猪NMB和受体基因分别注册GenBank(EU375564和EU670045)。

cDNA Cloning and Tissue Distribution of Porcine Neuromedin B and Its Receptor

The coding sequence of porcine Neuromedin B and its receptor gene were amplified based on the in silico sequence information in the present study.DNA sequencing and bioinformatic analysis showed that the porcine Neuromedin B gene was 567 bp long,has one open reading frame of 366 bp and encodes 121 amino acids.The length of Neuromedin BR gene was 1194 bp,which contain-ing one open reading frame of 1173 bp and encoding 390 amino acids.Compared to bovine,human,mouse and rat,the nucleotide homology of porcine Neuromedin B was 87.1%,85.2%,78.1% and 76.6%,respectively.The homology of amino acid sequence was 81%,74.4%,70.2% and 70.1%,respectively.As for the gene of porcine Neuromedin BR,the nucleotide homology was 91%,89%,84.2% and 83.6%,while the amino acid sequence homology was 92.3%,90.3%,86.9% and 86.4%,respectively.Semi-quantitative RT-PCR analysis showed that the neuromedin B gene ubiquitously expressed in various tissues tested,while the gene of Neuromedin BR preferentially expressed in the brain.The sequences of porcine Neuromedin B and its receptor gene were submitted to the GenBank(EU375564 and EU670045).