| 松口蘑SRAP反应体系的优化 |
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| 引用本文： | 吴松权,朴炫春,全雪丽,金美玉.松口蘑SRAP反应体系的优化[J].辽宁林业科技,2009(5):13-14. |
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| 作者姓名： | 吴松权 朴炫春 全雪丽 金美玉 |
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| 作者单位： | 延边大学,农学院,吉林,龙井,133400 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 以松口蘑为材料，建立了松口蘑SRAP反应的优化体系。即在20μL反应体积中，模板DNA 10ng，引物浓度0．3μmol／L，dNTP 250mmol／L，MgCl 22．5mmol／L，Taq DNA聚合酶0．5U，1×Buffer；反应程序为：94℃预变性5min，94℃变性1min，35℃退火1min，72℃延伸1min，共5个循环；94℃变性1min，50℃退火1min，72℃延伸1min，共35个循环：72℃延伸5min。
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| 关 键 词： | 松口蘑 SRAP 条件优化 |
Optimization of SRAP reaction systems for Tricholoma matsutake |
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| WU Song-quan,PIAO Xuan-chun,QUAN Xue-li,JIN Mei-yu.Optimization of SRAP reaction systems for Tricholoma matsutake[J].Liaoning Forestry Science and Technology,2009(5):13-14. |
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| Authors: | WU Song-quan PIAO Xuan-chun QUAN Xue-li JIN Mei-yu |
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| Institution: | College of Agriculture;Yanbian University;Longjing 133400;China |
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| Abstract: | Optimized SRAP reaction systems for Tricholoma matsutake were established. Namely, in the 20μL reaction volume, template DNA was 10ng, primer concentration 0.3 μmol/L, dNTP 250mmol/L, MgCl2 2.5mmol/L, Taq DNA polymerase 0.5 U and 1×Buffer. Reaction procedttres were that： predegenerating 5 min at 94℃, degenerating 1 min at 94℃, annealing 1 min at 35℃ and extending 1 min at 72℃, and 5 cycles were included; degenerating 1 rain at 94℃, annealing 1 min at 50℃ and extending 1 rain at 72℃, and 35 cycles were included; extending 5 rain at 72℃. |
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| Keywords: | SRAP |
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