| 异源多角体蛋白包装对重组家蚕核型多角体病毒感染性的影响 |
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| 引用本文： | 季平,何家禄.异源多角体蛋白包装对重组家蚕核型多角体病毒感染性的影响[J].中国农业科学,2001,34(1):111-114. |
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| 作者姓名： | 季平 何家禄 |
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| 作者单位： | 中国农业科学院蚕业研究所, |
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| 基金项目： | 国家自然科学基金资助项目(39770575);江苏省科委科技发展计划(BJ95138) |
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| 摘 要： | 将 Ac MNPV的 polh基因克隆到缺失 polh基因的 Bm NPV(API4)基因组中 ,得到被 Ac MNPV多角体蛋白包埋并可表达慈菇蛋白酶抑制剂的重组病毒 Bm NPV(OAc- API4)。发现它的芽生型病毒能感染家蚕细胞和经皮下注射感染家蚕幼虫 ,但包埋型病毒不能经口感染家蚕幼虫。
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| 关 键 词： | 家蚕核型多角体病毒 异源多角体蛋白基因 包埋型病毒 经口感染 PCR检测 |
| 文章编号： | 0578-1752(2001)01-0111-04 |
| 修稿时间： | 1999年11月29 |
Infectivity of the Recombinant Bombyx mori Nuclear Polyhedrosis Virus Containing Heterogeneous Polyhedrin |
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| JI Ping,HE Jia lu.Infectivity of the Recombinant Bombyx mori Nuclear Polyhedrosis Virus Containing Heterogeneous Polyhedrin[J].Scientia Agricultura Sinica,2001,34(1):111-114. |
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| Authors: | JI Ping HE Jia lu |
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| Abstract: | The polyhedrin gene(polh) of Autographa californica multicapsid nuclear polyhedrosis virus(AcMNPV) was cloned into the recombinant BmNPV(API4) genome which the polyhedrin gene of Bombyx mori nuclear polyhedrosis virus(BmNPV) was replaced by API4. And a recombinant BmNPV(OAc API4) was obtained. The recombinant virus was able to produce AcMNPV polyhedrin protein and expressed the Arrowhead proteinase inhibitor. The budded virus of the recombinant BmNPV(OAc API4) was able to infect BmN cells, and silkworm larvae using injection while per os inoculation with occluded virus couldn't infect the larvae. It was indicated that per os inoculation of NPV might be concerned with the type of polyhedrin proteins. |
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| Keywords: | Bombyx mori nuclear polyhedrosis virus (BmNPV) Heterogeneous polyhedrin gene Occluded virus particle Per os inoculation PCR detection |
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