Caffeine as main interfering compound in radioimmunoassay of aflatoxin B1 in coffee samples |
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| Authors: | P Rauch I Víden T Davídek J Velísek L Fukal |
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| Institution: | Institute of Chemical Technology, Prague, Czechoslovakia. |
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| Abstract: | The content of caffeine in coffee extracts prepared for radioimmunoassay of aflatoxin B1 was determined by gas chromatography. The extracts from coffee beans and decaffeinated coffee contained 1.76-4.60 and 0.71-0.85 g caffeine/kg, respectively. These concentrations of caffeine caused false results in radioimmunoassay of aflatoxin B1 in the range 1.0-2.8 micrograms/kg for coffee beans and 0.3-0.4 micrograms/kg for decaffeinated coffee. |
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