| 蘑菇分离物的DNA指纹鉴别 |
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| 引用本文： | 罗信昌.蘑菇分离物的DNA指纹鉴别[J].中国食用菌,2001,20(3):3-4. |
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| 作者姓名： | 罗信昌 |
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| 作者单位： | 华中农业大学, |
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| 基金项目： | 国家自然科学基金39770025；高等学校博士学科点专项基金资助项目部分内容 |
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| 摘 要： | 按照植物理学原理，蘑菇分离物应依据柯赫法则（Koch's postulate)回接，在一定条件下，人工诱用蘑菇）难以人工诱发子实体，即使能形成子实体也费时费力，本文介绍DNA指纹鉴别技术，即运用RAPD－PCR技术制成分离物及对应子实体指纹图谱，分析它们间DNA同源性及遗传相似度以鉴别分离物真的，探求快速，简便，有效的鉴别方法。
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| 关 键 词： | 蘑菇 分离物 RAPD－PCR DAN图谱 指纹鉴别 |
| 文章编号： | 1003—8310(2001)03—0003—02 |
| 修稿时间： | 2001年3月15日 |
DNA Fingerprinting Identifi Cation of Isolate in Mushrooms |
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| LUO Xin-chang.DNA Fingerprinting Identifi Cation of Isolate in Mushrooms[J].Edible Fungi of China,2001,20(3):3-4. |
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| Authors: | LUO Xin-chang |
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| Abstract: | The isolate of mushrooms should be identified by back inoculation,artificial induce sporophore formation under adapted condition according to Koch's postulate. However,it is very difficult to form sporophore by artificial inducing in some species,i.g.ectomycorrhizal mushrooms.Even if sporophore formation,it would take a long time and lots of efforts.Isolate would be identified by application PAPD-PCR technique,to make DNA band,analysis DNA homogeneity,DNA similarity coefficient between isolate and sporophore,which is of speed,handy and availble for characterization isolate of mushroom. |
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| Keywords: | Mushrooms Isolate RAPD-PCR DNA band |
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