番茄早疫病菌抗嘧菌酯突变体培养特性研究

通过对番茄早疫病菌抗、感嘧菌酯菌株的培养基营养条件、pH以及温度的改变,了解抗性菌株的生物学特性。结果表明:抗、感菌株之间的营养利用情况不同,敏感亲本在以可溶性淀粉为碳源和以蛋白胨为氮源的培养基中菌丝生长最快,而抗性菌株在葡萄糖和牛肉膏的培养基中生长最快;对pH值的要求也略有差别:抗、感菌株均在pH值为6、7时生长较迅速,抗性突变体在pH值为7时生长最快而亲本菌株在pH值为6时生长最快。二者在不同温度下生长情况基本相似:在4～30℃温度范围内菌丝均能生长,以20～25℃生长最快。其中在4～25℃之间菌丝生长速度随温度升高而加快;在25～30℃之间则随温度升高而减慢,在35℃几乎不生长,各温度下敏感亲本菌丝生长速度均比抗性突变体快。

Study on Culture Conditions of Resistant Strains of Alternaria solani to Azoxystrobin

The research aimed to understand the biological characteristics of Alternaria solani resistant and sensitive strains against azoxystrobin by changing the medium nutritional conditions,pH and temperature.The results showed that the usage of C and N source was different between the resistant and sensitive strains.The mycelium grew fastest when the resistant strains cultured on the medium in which glucose acted as C source and beef extract acted as N source,while the sensitive parent strains grew fastest on the medium of soluble starch as C source and peptone acted as N source.The most proper pH for the pathogen growth was a little different.The favorable pH for the resistant strains was 7 and that of the sensitive parent strains was 6.The growth of resistant and sensitive strains was basically similar at different temperatures.They all could grow at 4～ 30℃and 20～25℃ was the proper temperature to grow.Mycelium growth rate rise at 4～25℃ and fall at 25～30℃.They stopped growing at 35℃,the mycelium of sensitive parent strains grew faster than that of resistant strains at all temperatures.