| 拟南芥DREB2A基因的克隆及植物荧光表达载体的构建 |
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| 引用本文： | 段红英,丁笑生.拟南芥DREB2A基因的克隆及植物荧光表达载体的构建[J].华北农学报,2008,23(3). |
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| 作者姓名： | 段红英 丁笑生 |
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| 作者单位： | 河南师范大学,生命科学学院,河南,新乡,453007 |
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| 基金项目： | 河南省教育厅自然科学基金
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河南师范大学校科研和教改项目 |
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| 摘 要： | 以拟南芥幼苗总RNA为模板,采用RT-PCR技术扩增到DREB2A基因,并将其克隆到植物表达载体pCAM-BIA1304中CaMV 35 S启动子与poly(A)终止子之间。酶切鉴定及测序列结果都表明,成功构建了植物表达载体pCAMBIA1304-DREB2A。另外,获得了携带DREB2A基因的根瘤农杆菌菌株,为以后转基因植物工作奠定了基础。
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| 关 键 词： | 拟南芥 DREB2A RT-PCR 植物表达载体 根瘤农杆菌 |
Cloning of Arabidopsis thaliana DREB2A Gene and Its Construction of Its Plant Fluorescent Expression Vector |
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| DUAN Hong-ying,DING Xiao-sheng.Cloning of Arabidopsis thaliana DREB2A Gene and Its Construction of Its Plant Fluorescent Expression Vector[J].Acta Agriculturae Boreali-Sinica,2008,23(3). |
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| Authors: | DUAN Hong-ying DING Xiao-sheng |
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| Abstract: | In the paper,total RNA was extracted from Arabidopsis thaliana seedlings and was used as template to amplify the DREB2A gene by RT-PCR.The gene fragment was subsequently cloned into plant expression vector pCAMBIA1304,located between CaMV35S promoter and poly(A)terminator.Restriction enzyme analysis and DNA sequencing confirmed that the plant expression vector pCAMBIA1304-DREB2A was successfully construed.In addition,the strain of Agrobacterium tumefaciens carrying DREB2A gene were obtained,providing the foundation for transgenic study of plants. |
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| Keywords: | Arabidopsis thaliana DREB2A RTPCR Plant expression vector Agrobacterium tumefaciens |
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