Effect of cumulus cells and sperm concentration on fertilization and development of pig oocytes |
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| Authors: | R Li Y Liu HS Pedersen H Callesen |
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| Affiliation: | 1. Department of Animal Science, Aarhus University, Tjele, Denmark;2. Animal, Dairy, and Veterinary Sciences Department, Utah State University, Logan, UT, USA |
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| Abstract: | Effect of sperm concentrations and cumulus cells (CCs) on porcine IVF was re‐evaluated using current improved IVM and IVC system. Our results showed that both CCs and sperm concentration had significant effect on penetration rate, frequency of polyspermy and embryonic development. The best IVF results were obtained with oocytes with CCs fertilized with 0.5 × 105 sperm/ml. Such an IVP system works on both sow and gilt oocytes. |
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| Keywords: | IVF polyspermy post‐pubertal pronuclear formation swine |
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