Label‐free proteome of water buffalo (Bubalus bubalis) seminal plasma |
| |
| Authors: | Mayara F Brito Patrícia A Auler Guilherme C Tavares Cristiana P Rezende Gabriel M F Almeida Felipe L Pereira Carlos A G Leal Arlindo de Alencar Moura Henrique C P Figueiredo Marc Henry |
| |
| Institution: | 1. Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;2. AQUAVET – Laboratory of Aquatic Animal Diseases, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;3. AQUACEN – National Reference Laboratory for Aquatic Animal Diseases, Ministry of Agriculture, Livestock and Food Supply, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;4. Department of Animal Science, Universidade Federal do Ceara, Fortaleza, Brazil |
| |
| Abstract: | The study aimed to describe the Bubalus bubalis seminal plasma proteome using a label‐free shotgun UDMSE approach. A total of 859 nonredundant proteins were identified across five biological replicates with stringent identification. Proteins specifically related to sperm maturation and protection, capacitation, fertilization and metabolic activity were detected in the buffalo seminal fluid. In conclusion, we provide a comprehensive proteomic profile of buffalo seminal plasma, which establishes a foundation for further studies designed to understand regulation of sperm function and discovery of novel biomarkers for fertility. MS data are available in the ProteomeXchange with identifier PXD003728. |
| |
| Keywords: | buffaloes protein semen shotgun |
|
|
