链霉菌株Streptomyces sp. FXP04对水稻种子萌发和幼苗生长的影响

为了明确链霉菌株Streptomyces sp. FXP04对水稻的促生效果,以前期筛选到的链霉菌株Streptomyces sp. FXP04为供试菌株,采用发酵液浸种和菌液喷洒土壤的方法,研究了该菌株生长稳定期的发酵液和菌液对水稻种子萌发和幼苗生长的影响。结果如下,培养皿试验中,Streptomyces sp. FXP04发酵液原液和LB培养液原液（对照）都抑制种子萌发,1/50倍发酵液显著促进种子萌发,而1/10倍和1/100倍发酵液对种子萌发没有显著影响。盆栽试验中,1/50倍和1/100倍的Streptomyces sp. FXP04菌液都促进了水稻种子萌发,增加了水稻幼苗株高、主根长、干重、根总长度、须根数、根表面积和根总体积,并且1/50倍菌液的促生效果和1/100倍菌液无显著差异。研究结果表明,链霉菌株Streptomyces sp. FXP04对水稻种子萌发和幼苗生长有促进作用,可作为水稻的候选促生菌株开展后续研究和应用。

Effects of Streptomyces sp. FXP04 on Seed Germination and Seedling Growth of Rice

In order to determine the growth promoting effects of Streptomyces sp. FXP0404 on rice growth, the Streptomyces sp. FXP04 isolated in our previous experiments was selected as the test strain to study the effects of fermented liquid and cell suspension of Streptomyces sp. FXP04 on the seed germination and seedling growth of rice, using the methods of soaking seeds with fermented liquid and spraying soil with cell suspension. The results showed that both the original fermented liquid and LB liquid (control) inhibited seeds germination in the culture dish. Compared to water treatment, the rice seed germination was significantly increased in the treatment of 1/50 times fermented liquid, however, there were no significant effect on seed germination in the 1/10 and 1/100 times fermented liquid treatments. In the pot experiment, both 1/50 and 1/100 times cell suspension of Streptomyces sp. FXP04 promoted the germination of rice seeds, increased the plant height, main root length, dry weight, total root length, fibrous root number, root surface area and total root volume of rice seedlings, however, there was no significant difference between 1/50 and 1/100 times of Streptomyces sp. FXP04 cell suspension. The results demonstrated that Streptomyces sp. FXP04 could promote the seed germination and seedling growth of rice, and could be used as a candidate plant growth-promoting rhizobacteria on rice for further research and application.