采用MiSeq测序技术分析3种飞虱中肠内容物的菌群结构

运用MiSeq测序技术比较了褐飞虱(Nilaparvata lugens St?l,BPH)、灰飞虱(Laodelphax striatella Fallén,SBPH)和白背飞虱(Sogatella furcifera Horvath,WBPH)体内中肠细菌群落结构的异同。结果显示:3种飞虱共获得可用于分析的有效序列3.44×10⁵条,褐飞虱、灰飞虱和白背飞虱在3%水平和6%水平分别获得76、103、61和49、68、42个操作分类单元(operational taxonomic unit,OTU)。灰飞虱体内约90%的中肠细菌属于F-proteobacteria-unclassified;Arsenophonus是褐飞虱体内的优势菌,约占总量的70%;在白背飞虱中Halomonas、Candidatus_Cardinium和Arsenophonus三种菌含量都比较高,分别占35%、20%和15%。虽然3种飞虱从共同的祖先分化,但其中肠内细菌微生物群落多样性、丰富度仍存在一定程度的差异。

Diversities of microbial communities in midgut of 3 planthoppers species by MiSeq

In this study, we successfully demonstrated that MiSeq was a powerful approach for investigating the bacterial communities in midgut of brown planthopper (BPH) Nilaparvata lugens St?l, white-backed planthopper (WBPH) Sogatella furcifera Horvath and small brown planthopper(SBPH)Laodelphax striatellus(Fallen). For all three planthoppers, 3.44×10 ⁵ effective sequences were selected and utilized for the bacterial diversity and abundance analysis. In total, 76, 103, and 61 operational taxonomic units were obtained at 3% distance cutoff, 49, 68 and 42 at 6% distance cutoff in the BPH, SBPH and WBPH, respectively. Nearly 90% of the sequences in the SBPH were assigned into F-proteobacteria-unclassified. In the other two samples, Arsenophonus was the dominant genus in BPH, accounting for about about 70% sequences, while in WBPH, Halomonas, Candidatus_Cardinium and Arsenophonus were 3 dominant bacterias, accounting for about 35%, 20% and 15%, respectively. Though they were evolved from the common ancestor, the microbial community diversity, richness, dominant bacteria function of endosymbiont in three planthoppers were different.