葡萄MPT基因家族鉴定与表达分析

线粒体磷酸转运体(mitochondrial phosphate transporter, MPT)基因家族是位于线粒体内膜上的一种重要的功能蛋白,负责将重要代谢底物无机磷酸通过线粒体内膜从细胞质转移到线粒体基质中,在细胞维持正常功能中起到了关键的生理作用。本研究利用生物信息学工具,系统地分析了葡萄MPT基因家族成员的进化关系、基因结构、蛋白质理化性质和保守基序,以及组织特异性表达和同源基因的相关性。结果表明,葡萄全基因组中鉴定出64个MPT家族基因。根据系统发育树可以分为7组,分布于19条染色体上,其中两对基因存在串联复制现象。保守基序分析表明,所有MPT家族成员都含有保守的Mito_carr结构域。组织特异性表达表明,VvMPT基因在果皮、花、叶中高表达。同源基因分析表明,葡萄VvMPT基因家族可能在花器官发育、维持离子浓度中起到了重要作用。

Identification and expression analysis of grape MPT gene family

The mitochondrial phosphate transporter (MPT) gene family is an important functional protein located on the innner membrane of the nitochondria, transferring important metabolic substrate inorganic phosphate from the cytoplasm to the mitochondrial matrix through mitochondrial inner membrane and playing a key physiological role in maintaining the normal function of cells. Using bioinformatics tools, the phylogenetic evolution, structure, protein physicochemical properties and conserved motifs of grape MPT gene family members were systematically analyzed, as well as the tissue-specific expression and association of homologous gene. The results showed that sixty-four possible MPT family genes were identified in the whole genome of grapes, which could be divided into 7 groups according to the phylogenetic tree, distributed on 19 chromosomes, and two pairs of genes had a tandem replication phenomenon. Conservative motif analysis showed that all MPT family members contained the conserved domain of Mito_carr. Tissue-specific expression showed that VvMPT gene was highly expressed in peels, flowers and leaves. Homologous gene analysis showed that the VvMPT gene family of grapes might play an important role in the development of floral organs and maintenance of ion concentration.