红芽芋脱毒试管芋诱导及植株再生

为解决红芽芋脱毒苗移栽成活率低、种苗易分化等问题,以江西地方名优芋品种‘铅山红芽芋’为材料,开展茎尖脱毒、试管芋诱导及植株再生的研究。结果表明,芋芽剥取0.2～1.0 mm茎尖接种于MS+2.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA+3%蔗糖培养基中可诱导成苗;通过RT-PCR分子检测,0.3 mm以下茎尖培养的试管苗中未发现芋花叶病毒(DsMV);脱毒苗继代增殖最佳诱导培养基为MS+3.0mg·L^-1 6-BA+0.5 mg·L^-1 NAA+3%蔗糖,脱毒苗试管芋诱导的最佳培养基为MS+1.0 mg·L^-1 6-BA+0.5 mg·L^-1 NAA+8%蔗糖,最佳培养条件为温度25℃,光照54μmol·m^-2·s^-1,光周期14 h·d^-1;脱毒试管芋的最佳移栽驯化基质为草炭︰蛭石=2︰1;脱毒试管芋移栽后,成活率达97.71%,生长中未出现分化现象。脱毒芋相比未脱毒芋增产4...

Induction and Plant Regeneration of Virus-free Microtuber in Red Bud Taro

In order to solve the low survival rate and high differentiation rate of virus-free seedling of red bud taro seedlings in the process of transplanting，in this study，with taking the most famous and high-quality local cultivars of Yanshan red bud taro（Colocasia esculenta）in Jiangxi Province as experimental material，the detoxification culture of taro stem tip，molecular detection of test-tube plantlets and microtuber induction were carried out. The results showed that seedlings in vitro could be induced from 0.2–1.0 mm taro buds inoculated in MS + 2.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 3% sucrose medium，and RT-PCR molecular detection indicated that the seedlings induced from stem tip under 0.3 mm diameter in culture could not carry virus（DsMV）. The optimal medium for the induction of secondary proliferation of virus-free seedlings was MS + 3.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 3% sucrose，the optimal medium for the induction of virus-free cormel was MS + 1.0 mg • L-1 6-BA + 0.5 mg • L-1 NAA + 8% sucrose，the optimal culture virus-free seedlings and taro corm conditions were 25 ℃，54 μmol • m-2 • s-1，14 h • d-1，the optimal substrates for transplanting and acclimation of was peat︰vermiculite（2︰1），and the survival rate was 97.71%. Compared with the contrast，the yield of virus-free taro increased 43.14% and there were significant differences in nutritional characters between them. The formation of virus-free cormel and the establishment of a plant regeneration system provide a new way for the rapid propagation of healthy seedlings of red bud taro.