山豆根多糖对猪圆环病毒2型感染小鼠体内外炎性因子分泌水平的影响

旨在探究山豆根多糖对猪圆环病毒2型（porcine circovirus 2,PCV2）感染小鼠体内外炎性因子分泌水平的影响。通过PCV2感染小鼠脾淋巴细胞建立体外炎症模型,用不同浓度（100、200、400 μg/mL）的山豆根多糖作用细胞,采用ELISA法测定细胞分泌IL-1β、IL-8、MCP-1水平和细胞内COX-1活性;利用PCV2体内感染昆明种小鼠建立氧化胁迫模型后,腹腔注射低、中、高浓度100、200、400 mg/（kg·BW）]的山豆根多糖,应用ELISA法测定小鼠脾脏、肺脏组织和血清中炎性因子IL-1β、IL-8、MCP-1分泌水平和COX-1活性。结果显示,与PCV2感染模型细胞相比,经不同浓度山豆根多糖处理的小鼠脾淋巴细胞中炎性因子IL-1β、IL-8、MCP-1水平和细胞内COX-1活性均不同程度降低,其中,400 μg/mL的山豆根多糖作用最佳（P<0.01）;利用不同浓度的山豆根多糖腹腔注射PCV2感染小鼠后,均能抑制PCV2感染小鼠的脾脏、肺脏组织和血清中炎性因子IL-1β、IL-8、MCP-1分泌水平和COX-1的活性,其中,高浓度400 mg/（kg·BW）]的山豆根多糖作用最佳（P<0.01）。综上提示,山豆根多糖能够抑制PCV2感染小鼠炎性因子分泌,从而发挥抗炎作用。

In Vivo and In Vitro Effect of Sophora subprosrate Polysaccharide on the Secretion of Inflammatory Factors in Mice Infected with Porcine Circovirus Type 2

The aim of this study was to explore the effect of Sophora subprosrate polysaccharide on the secretion of inflammatory factors in vivo and in vitro in mice infected with porcine circovirus 2 （PCV2）. An in vitro inflammation model was established after mouse splenic lymphocytes were infected with PCV2, the cells were treated with Sophora subprosrate polysaccharide at different concentrations （100, 200, 400 μg/mL）, and the secreted IL-1β, IL-8, MCP-1 levels by lymphocytes and intracellular COX-1 activity were measured by ELISA method; an oxidative stress model was established after infecting Kunming mice with PCV2 in vivo, intraperitoneal injections of low, medium and high concentrations 100, 200, 400 mg/（kg·BW）] Sophora subprosrate polysaccharide were given and ELISA method was used to determine the secretion level of inflammatory factors IL-1β, IL-8, MCP-1 and activity of COX-1 in mouse spleen, lung tissue and serum. The results showed that, compared with PCV2 infection model cells, the secreted inflammatory factors IL-1β, IL-8, MCP-1 and intracellular COX-1 activity in mouse splenic lymphocytes treated with different concentrations of Sophora subprosrate polysaccharide were reduced at different levels, and 400 μg/mL Sophora subprosrate polysaccharide had the best effect （P<0.01）; after intraperitoneal injection of PCV2 with different concentrations of Sophora subprosrate polysaccharide in mice, it could inhibit the spleen, lung tissue and serum levels of inflammatory factors IL-1β, IL-8, MCP-1 and COX-1 activity, and the high concentration 400 mg/（kg·BW）] Sophora subprosrate polysaccharide had the best effect （P<0.01）. In summary, Sophora subprosrate polysaccharide can inhibit the secretion of inflammatory factors in mice infected with PCV2, thereby exerting an anti-inflammatory effect.