Abstract: | After infection of mice with hepatitis virus MHV3, the RNA in the liver undergoes changes. The fraction extracted with phenol at 0 degrees C does not alter. However, the fraction extracted with hot phenol at elevated pH (60 degrees C, pH 8.3) shows a 16S peak on sucrose-density-gradient centrifugation. This fraction shows actinomycin D-resistant incorporation of C(14)-orotic acid in infected but not in control livers-possible evidence of the RNA nature of MHV3. |