| 丝虫抗原的分离及在鹿脑脊髓丝虫病诊断中的应用 |
| |
| 引用本文： | 陈立秀,陈同海,刘萍,王树志.丝虫抗原的分离及在鹿脑脊髓丝虫病诊断中的应用[J].经济动物学报,2001,5(2):17-22. |
| |
| 作者姓名： | 陈立秀 陈同海 刘萍 王树志 |
| |
| 作者单位： | 1. 无锡锡惠公园动物园,江苏,无锡,214035
2. 吉林农业大学,长春,130118 |
| |
| 摘 要： | 首次报道应用牛腹腔丝虫成虫破碎后 ,经SephadexG 2 0 0柱层析获得CG1 抗原 ,进行酶联免疫吸附试验 (ELISA)免疫诊断鹿脑脊髓丝虫病的新方法。结果证明 ,该种方法特异性强 ( 87 1 % ) ,敏感性高 ( 1∶640 0 )。通过对 380头假定健康鹿ELISA结果与虫检结果对比证明 ,2种诊断方法符合率为 1 0 0 %。ELISA法比虫检法检出率高 3 2 % ,因此 ,可以用ELISA代替虫检诊断鹿脑脊髓丝虫病
|
| 关 键 词： | 鹿 脑脊髓丝虫病 丝虫抗原(ELISA) 免疫诊断 |
| 文章编号： | 1007-7448(2001)02-0017-06 |
| 修稿时间： | 2000年5月8日 |
Isolation of Cerebrospinal Filariasis Antigen and Its Utieization in Diagnosis of Cerebrospinal Filariasis in Deer |
| |
| CHEN Li xiu ,CHEN Tong hai ,LIU Ping ,WANG Shu zhi.Isolation of Cerebrospinal Filariasis Antigen and Its Utieization in Diagnosis of Cerebrospinal Filariasis in Deer[J].Journal of Economic Animal,2001,5(2):17-22. |
| |
| Authors: | CHEN Li xiu CHEN Tong hai LIU Ping WANG Shu zhi |
| |
| Institution: | CHEN Li xiu 1,CHEN Tong hai 1,LIU Ping 2,WANG Shu zhi 2 |
| |
| Abstract: | A new ELISA method for diagnosis of cerebrospinal filariasis with sephadex G 200 separated antigen CG 1 was developed. The results showed that CG 1Ag had high specifity(87.1%) and sensitivity(1∶6400).The positive coincide between ELISA and worm detection were 100%,and the result of ELISA was 3.2% higher than that of worm detection,suggesting that CG 1Ag instead of worm detection could achieve high position rate. |
| |
| Keywords: | deer cerebraspinal filariasis ELISA immune diagnosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
