| 番茄EST-SSR标记PCR反应体系的优化 |
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| 引用本文： | 韩明利,侯丽霞,崔娜,王施慧,于志海,郭彩杰.番茄EST-SSR标记PCR反应体系的优化[J].北方园艺,2011(16):142-144. |
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| 作者姓名： | 韩明利 侯丽霞 崔娜 王施慧 于志海 郭彩杰 |
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| 作者单位： | 山东省农业科学院蔬菜研究所,山东省设施蔬菜生物学重点实验室,国家蔬菜改良中心山东分中心,山东济南250100;沈阳农业大学生物科学技术学院,辽宁沈阳110161;山东省农业科学院蔬菜研究所,山东省设施蔬菜生物学重点实验室,国家蔬菜改良中心山东分中心,山东济南250100;沈阳农业大学生物科学技术学院,辽宁沈阳,110161 |
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| 基金项目： | 国家“863”计划资助项目(2006AA100108-3-1); 山东省农业良种工程资助项目(2010LZ006-01) |
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| 摘 要： | 应用L16 (44)正交实验优化番茄EST-SSR标记PCR反应体系.结果表明:20 μL体系中各反应物最适浓度为:DNA模板45 ng/20μL,引物0.75 μmol/L,Mg2+ 2.0 mmol/L,dNTPs0.4 mmol/L,Taq DNA酶0.5 U/20μL,且Mg2+对该标记反应体系影响最大.利用5对EST-SSR引物及P1、P2基因组DNA验证优化体系的可靠性,为该标记在番茄基因组分子标记辅助育种提供了试验依据.
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| 关 键 词： | 番茄 EST-SSR 体系优化 PCR |
Optiminzation of EST-SSR Maker PCR System on Tomato |
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| HAN Ming-li
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HOU Li-xia
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CUI Na
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WANG Shi-hui
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YU Zhi-hai
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GUO Cai-jie.Optiminzation of EST-SSR Maker PCR System on Tomato[J].Northern Horticulture,2011(16):142-144. |
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| Authors: | HAN Ming-li HOU Li-xia CUI Na WANG Shi-hui YU Zhi-hai GUO Cai-jie |
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| Institution: | HAN Ming-li1,2,HOU Li-xia1,CUI Na2,WANG Shi-hui1,YU Zhi-hai2,GUO Cai-jie2 (1.Institute of Vegetable Research Center,Shandong Academy of Agricultural Sciences,Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province,National Improvement Center for Vegetable,Shandong Branch,Jinan,Shandong 250100,2.College of Biological Science and Technology,Shenyang Agricultural University,Shenyang,Liaoning 110161) |
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| Abstract: | L16(44) orthogonal design was used in the EST-SSR Maker PCR system optimization of tomato.The results showed that the best PCR system was:45 ng/20μL DNA template,0.75 μmol/L primer,2.0 mmol/L Mg2+,0.4 mmol/L dNTPs,0.5 U/20μL Taq DNA polymerase,and the effect of the primer concentration was the greatest for this system.The system had been verified by five pairs of primers and P1,P2 genome DNA,and provided experiments reference on molecular marker assisted selection of tomato. |
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| Keywords: | tomato EST-SSR system optimization PCR |
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