菜用大黄组培苗不定芽增殖能力比较研究

以5个欧洲菜用大黄品种‘RT’、‘RO'、‘RV’、‘R5’和‘R19'种子为试材,以组培苗茎尖为外植体,在MS+1.0 mg/L NAA+1.0 mg/L 6-BA+5.3 g/L琼脂+30 g/L蔗糖培养基上进行连续24 d的离体培养,研究不同欧洲菜用大黄品种组培苗不定芽增殖能力差异.结果表明:5个品种的不定芽再生方式可以分为基部愈伤组织萌发再生不定芽和腋芽萌发再生不定芽.各品种间不定芽再生能力差异显著,‘RT'的再生不定芽增殖速度最快,增殖系数最大,为3.71,‘R19'的再生不定芽增殖速度最慢,增殖系数最小,为1.96;根据各品种增殖系数连续变化曲线得出,菜用大黄合理的继代周期为24 d.

Study on Regeneration of Adventitions Bud of Tissue Culture Seedling of Different Rhubarb Cultivars

Five rhubarb cultivars seeds of ‘RT’,‘RO’,‘RV’,‘R5’and’R19’ introduced from Europe were used as test material,and shoot tip of tissue culture seedling as explants cultured on MS medium supplemented with 1.0 mg/L NAA and 1.0 mg/L 6-BA and 5.3 g/L agar and 30 g/L sucrose,to investigated the regeneration difference of tissue culture seedling of five cultivars.The results showed that two different patterns of shoot proliferation were noted,i.e.caespitose shoots proliferating from callus of explants and axillary shoot bourgeoning from explants;significant difference in regenerative ability was observed,with cultivar ’RT’ showed the highest proliferation coefficient was 3.71 and ‘R19’ presenting the lowest proliferation coefficient was 1.96;the variation curve of proliferation coefficient suggested that subculture for multiplication can be conducted in every 24 days to establish a micro-propagation system.