| 丝瓜SRAP反应体系的建立与优化 |
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| 引用本文： | 李莲芳,郭爽,林鉴荣.丝瓜SRAP反应体系的建立与优化[J].北方园艺,2011(15):160-162. |
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| 作者姓名： | 李莲芳 郭爽 林鉴荣 |
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| 作者单位： | 广州市农业科学研究院,广东广州,510308;广州市农业科学研究院,广东广州,510308;广州市农业科学研究院,广东广州,510308 |
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| 基金项目： | 广州市科技局资助项目(2010Z1-E381) |
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| 摘 要： | 以丝瓜基因组DNA为模板,采用正交实验设计L16 (45),在4个水平上对影响丝瓜SRAP反应的Taq酶、Mg2+、模板、dNTP及引物等5个因素进行了优化,建立了丝瓜SRAP-PCR的最佳反应体系.利用18份丝瓜自交系材料来验证此反应体系,均可扩增出清晰、可辨的条带,可见该反应体系较稳定,适用于丝瓜SRAP标记的扩增.
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| 关 键 词： | 丝瓜 SRAP 体系优化 |
Establishment and Optimization of SRAP Amplification System in Loofah |
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| LI Lian-fang
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GUO Shuang
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LIN Jian-rong.Establishment and Optimization of SRAP Amplification System in Loofah[J].Northern Horticulture,2011(15):160-162. |
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| Authors: | LI Lian-fang GUO Shuang LIN Jian-rong |
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| Institution: | LI Lian-fang,GUO Shuang,LIN Jian-rong(Guangzhou Academy of Agricultural Sciences,Guangzhou,Guangdong 510308) |
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| Abstract: | Loofah genom DNA was used for the PCR template to optimize loofah SRAP-PCR reaction system.Orthogonal experiments were designed to optimize the key parameters including Taq polymerase,Mg2+,template DNA,dNTPs,primer and so on.A SRAP-PCR system with good stability was established.The optimized SRAP-PCR system was tested by 18 loofah inbred lines and was steady and reliable. |
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| Keywords: | loofah SRAP-PCR system optimization |
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