| 国兰ISSR-PCR反应体系的建立与优化 |
| |
| 引用本文： | 刘菲,李萍,何俊荣,蒋彧,卓碧萍,孙淑霞.国兰ISSR-PCR反应体系的建立与优化[J].北方园艺,2011(17):136-139. |
| |
| 作者姓名： | 刘菲 李萍 何俊荣 蒋彧 卓碧萍 孙淑霞 |
| |
| 作者单位： | 西南交通大学,四川成都,610031;四川省农业科学院园艺研究所,四川成都,610066 |
| |
| 基金项目： | 四川省科技支撑计划资助项目(09ZC1745); 四川省育种工程花卉资助项目; 四川省财政厅“草本花卉”资助项目 |
| |
| 摘 要： | 采用正交设计与单因素结合法,对国兰ISSR-PCR反应体系中的4个因素(dNTPs、引物浓度、Mg2+、Taq DNA聚合酶)进行优化试验,结果用DPS软件进行分析.结果表明:各因素对PCR结果均有显著影响,其中Taq DNA聚合酶对反应的影响最大；筛选出了各反应因素的最佳水平,建立国兰ISSR-PCR的最佳反应体系(25μL)为:dNTPs 0.2mmol/L、引物1.0 μmol/L、Mg2+2.5 mmol/L、Taq DNA聚合酶1U.
|
| 关 键 词： | 国兰 分子标记 ISSR-PCR 反应体系 正交设计 |
Establishment and Optimization of ISSR-PCR Reaction System of Chinese Cymbidium |
| |
| LIU Fei
,
LI Ping
,
HE Jun-rong
,
JIANG Yu
,
ZHUO Bi-ping
,
SUN Shu-xia.Establishment and Optimization of ISSR-PCR Reaction System of Chinese Cymbidium[J].Northern Horticulture,2011(17):136-139. |
| |
| Authors: | LIU Fei LI Ping HE Jun-rong JIANG Yu ZHUO Bi-ping SUN Shu-xia |
| |
| Institution: | LIU Fei1,LI Ping1,HE Jun-rong2,JIANG Yu2,ZHUO Bi-ping2,SUN Shu-xia2(1.Southwest Jiaotong University,Chengdu,Sichuan 610031,2.Institute of Horticulture,Sichuan Academy of Agricultural Sciences,Sichuan 610066) |
| |
| Abstract: | Using the orthogonal design and single factor experiment to optimize ISSR-PCR amplification system of four factors(dNTPs,primer,Mg2+ and Taq DNA polymerase,) respectively.PCR results showed that each factor in different levels has significant effect on the result of PCR,the most remarkable factor was the quantity of Taq DNA polymerase.The optimum level of ISSR-PCR system for jujube was established.The total reaction system was 25 μL and contained 0.2 mmol/L dNTPs,1.0 μmol/L Primer,2.5 mmol/L Mg2+,1 U Taq DNA polymerase. |
| |
| Keywords: | Chinese cymbidium molecular marker ISSR-PCR reaction system orthogonal design |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
