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兰州百合多倍体诱导及鉴定
引用本文:刘静,赵庆芳,丁兰.兰州百合多倍体诱导及鉴定[J].北方园艺,2011(18):138-141.
作者姓名:刘静  赵庆芳  丁兰
作者单位:安康学院农学与生命科学院,陕西安康,725000;西北师范大学生命科学学院,甘肃兰州,730070
基金项目:甘肃省科技攻关计划资助项目(2GS042-A41-001-11)
摘    要:以兰州百合鳞片为试材,利用秋水仙碱溶液对兰州百合进行了人工多倍体诱导.结果表明:秋水仙碱最佳处理浓度和最佳处理时间分别为0.l%、24 h,细胞诱变率达60%.对获得的兰州百合多倍体与二倍体叶片气孔大小及密度进行测定,通过方差分析,气孔大小及密度差异达到极显著水平.对兰州百合多倍体和二倍体植株根尖染色体计数发现,二倍体植株所有细胞染色体数为2n=2x=24,多倍体植株中有的细胞染色体数为2n=2x=24,而有些细胞染色体数为2n=4x=48.

关 键 词:兰州百合  多倍体  秋水仙碱

Polyploid Iduction and Indentification of Lilium davidii var.unicolor(Hoog) Cotton
LIU Jing , ZHAO Qing-fang , DING Lan.Polyploid Iduction and Indentification of Lilium davidii var.unicolor(Hoog) Cotton[J].Northern Horticulture,2011(18):138-141.
Authors:LIU Jing  ZHAO Qing-fang  DING Lan
Institution:LIU Jing1,ZHAO Qing-fang2,DING Lan2 (1.College of Agriculture and Life Sciences,Ankang University,Ankang,Shaanxi 725000,2.College of Life Science,Northwest Normal Universtiy,Lanzhou,Gansu 730070)
Abstract:L.davidii var.unicolor(Hoog)Cotton scale was used as test material to inducted the polyploidy of L.davidii var.unicolorr(Hoog)Cotton using colchicine solution.The results showed that best concentration of colchcine was 0.1% in chromosome doubling induction of L.davidii var.unicolor(Hoog)Cotton.Under the concentration,the optimial time was 24 h,and the mutagenesis rate was 60%.Comparing the size and the number of stomata per unit leaf area of the leaf of diploid and the polyploidy plants,by the statistical a...
Keywords:Lilium davidii var  unicolor(Hoog) Cotton  polyploidy  colchicin  
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