| 植物生长调节剂对豆腐柴愈伤组织诱导的影响 |
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| 引用本文： | 程华,李琳玲,姜德志,袁红慧,程水源. 植物生长调节剂对豆腐柴愈伤组织诱导的影响[J]. 北方园艺, 2011, 0(18): 126-128 |
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| 作者姓名： | 程华 李琳玲 姜德志 袁红慧 程水源 |
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| 作者单位： | 经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000;黄冈师范学院化学与生命科学学院,湖北黄冈438000;经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000;黄冈师范学院化学与生命科学学院,湖北黄冈438000;经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000;黄冈师范学院化学与生命科学学院,湖北黄冈438000;经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000;黄冈师范学院化学与生命科学学院,湖北黄冈438000;经济林木种质改良与资源综合利用湖北省重点实验室,湖北黄冈438000;黄冈师范学院化学与生命科学学院,湖北黄冈438000 |
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| 基金项目： | 湖北省教育厅高校产学研合作重点资助项目(CXY2009B045); 湖北省自然科学基金资助项目(2008CDZ085) |
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| 摘 要： | 以湖北省罗田县野生豆腐柴叶片为材料,在MS培养基上附加不同浓度和比值的激素进行愈伤组织的诱导试验.结果表明:不同外源激素浓度及配比对外植体愈伤组织诱导率的表现不同,NAA比IBA更有利于豆腐柴愈伤组织的形成和生长；而低浓度6-BA更适于豆腐柴愈伤组织的分化与生长；外源激素的配比对愈伤组织诱导率有一定影响,浓度比为0.2的6-BA/NAA有利于豆腐柴愈伤的形成和生长；愈伤组织培养最终认为,诱导豆腐柴愈伤组织和生根的适宜培养基为0.2 mg/L 6-BA和1mg/L的NAA.
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| 关 键 词： | 豆腐柴 组织培养 愈伤组织 植物生长调节剂 |
Effect of Plant Growth Regulator on Callus Induction of Premna microphylla Turcz |
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| CHENG Hua , LI Lin-ling , JIANG De-zhi , YUAN Hong-hui , CHENG Shui-yuan. Effect of Plant Growth Regulator on Callus Induction of Premna microphylla Turcz[J]. Northern Horticulture, 2011, 0(18): 126-128 |
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| Authors: | CHENG Hua LI Lin-ling JIANG De-zhi YUAN Hong-hui CHENG Shui-yuan |
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| Affiliation: | CHENG Hua1,2,LI Lin-ling1,JIANG De-zhi1,YUAN Hong-hui1,CHENG Shui-yuan1,2 (1.Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories,Huanggang,Hubei 438000,2.College of Chemistry and Life Science,Huanggang Normal University,Hubei 438000) |
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| Abstract: | Premna microphylla leaves were used as explant in MS medium,in which added different concentration and concentration ratio of hormones for callus induction frequencies.The results showed that the CIF of exogenous hormones showed that NAA was more valuable in the formation and upgrowth of callus.Callus had more tissue differentiation and grew faster when the calluses were cultured with a low concentration of 6-BA.Exogenous hormones proportion had certain effection on calluses induction,it's beneficial to for... |
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| Keywords: | Premna microphylla Turcz tissue culture calluses plant growth regulator |
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