| '砀山酥'梨叶片再生体系的建立 |
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| 引用本文： | 付镇芳,孟颢光,张朝红,王跃进. '砀山酥'梨叶片再生体系的建立[J]. 北方园艺, 2011, 0(14): 98-101 |
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| 作者姓名： | 付镇芳 孟颢光 张朝红 王跃进 |
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| 作者单位： | 西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,陕西,杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西省农业分子生物学重点实验室,陕西,杨凌,712100 |
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| 基金项目： | 国家“863”计划资助项目(2007AA10Z182); 陕西省重点科技专项资助项目(2006kz05-G4) |
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| 摘 要： | 以‘砀山酥’梨(Pyrus bretschneidericv.Dangshansu)叶片为材料,研究基本培养基种类、植物生长调节物质浓度、抗氧化剂、碳源对再生的影响,以及不同浓度生长激素对再生芽生根的影响。结果表明:NN69培养基是叶片再生不定芽的最佳培养基,诱导不定芽的分化以培养基NN69+6-BA 3.0 mg/L+IBA 0.2 mg/L+琼脂6 g/L为佳;AgNO3比活性炭和PVP更利于叶片的诱导;与蔗糖相比山梨醇更适宜作为叶片再生的碳源,最佳浓度是10 mg/L,叶片再生频率最高为74.03%,平均再生芽数1.01。生根培养基以ASH+IBA 10 mg/L+间苯三酚80 mg/L+蔗糖15 g/L+琼脂6 g/L为佳,生根率高且根粗壮。
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| 关 键 词： | 梨 叶片再生 山梨醇 |
Primary Establishment of Plant Regeneration System of Pear Scion Cultivar 'Dangshansu' |
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| FU Zhen-fang,MENG Hao-guang,ZHANG Chao-hong,WANG Yue-jin. Primary Establishment of Plant Regeneration System of Pear Scion Cultivar 'Dangshansu'[J]. Northern Horticulture, 2011, 0(14): 98-101 |
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| Authors: | FU Zhen-fang MENG Hao-guang ZHANG Chao-hong WANG Yue-jin |
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| Affiliation: | FU Zhen-fang,MENG Hao-guang,ZHANG Chao-hong,WANG Yue-jin(College of Horticulture,Northwest Agricultural and Forestry University,Key Laboratory of Horticultural Plant Germplasm Resources Utilization in Northwest China,Ministry of Agriculture of China,Shaanxi Key Laboratory for Molecular Biology of Agriculture,Yangling,Shaanxi 712100) |
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| Abstract: | Pear scion cultivar 'Dangshansu' was chosed as material.The factors which include medium,plant growth regulators,antioxidant,and concentration of sorbitol that influence adventitious buds regeneration from leaf were examined systematically.The results showed that the optimal combination for leaf regeneration of pear cultivar 'Dangshansu' was NN69+6-BA 3.0 mg/L+IBA 0.2 mg/L+agar 6 g/L at the beginning of culture.AgNO3was more excellent than activated carbon and PVP.Compared with sugar as carbon sources of re... |
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| Keywords: | pear leaf culture sorbitol |
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