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沂州木瓜初代培养技术研究
引用本文:杨凤玲.沂州木瓜初代培养技术研究[J].北方园艺,2011(17):144-145.
作者姓名:杨凤玲
作者单位:临沂大学实验中心,山东临沂,276000
摘    要:利用沂州木瓜新长出10 d左右的嫩茎为外植体,采用不同消毒剂、灭菌时间、不同浓度6-BA对其进行初代培养试验.结果表明:沂州木瓜最佳消毒药剂及时间是70%酒精10 s+0.1%升汞2~4 min.当NAA浓度为0.05 mg/L时,沂州木瓜试管苗生长适宜的6-BA浓度为0.5 mg/L.

关 键 词:沂州木瓜  细胞分裂素  丛生芽  外植体

Studies on the Initial Culture Technology of Yizhou Pawpaw
YANG Feng-ling.Studies on the Initial Culture Technology of Yizhou Pawpaw[J].Northern Horticulture,2011(17):144-145.
Authors:YANG Feng-ling
Institution:YANG Feng-ling(Experiment Center,Linyi University,Linyi,Shandong 276000)
Abstract:The 10 days new buds of Yizhou pawpaw were used as the explants.These explants were cultured primarily with different disinfectors and different sterilizing time and different concentration of 6-BA.The results showed that it was the best for the explants to treat with 70% alcohol for 10 s and 0.1% HgCl2 for 2~4 min.When the concentration of NAA was 0.05 mg/L,the suitable concentration of 6-BA for Yizhou pawpaw test-tube plantlet growth was 0.5 mg/L.
Keywords:Yizhou pawpaw  cytokinin  multiple shoots  explant  
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