| 番茄中维生素E合成相关基因的VIGS载体构建及侵染 |
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| 引用本文： | 芦亮亮,傅达奇,刘海萍,王晓辉,罗云波,朱本忠,左进华.番茄中维生素E合成相关基因的VIGS载体构建及侵染[J].北方园艺,2011(16):139-142. |
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| 作者姓名： | 芦亮亮 傅达奇 刘海萍 王晓辉 罗云波 朱本忠 左进华 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083;中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目(30871741) |
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| 摘 要： | 以番茄为材料,采用RT-PCR技术从番茄果实中克隆了其体内维生素E合成相关基因—生育酚环化酶(Tocopherol cyclase VTE1)的部分序列,片段长420 bp,序列分析表明,该基因片段与番茄中生育酚环化酶cDNA序列同源性为95％,然后通过XhoⅠ、KpnⅠ 2个酶切位点连接VTE1片段和含PDS的pTRV2质粒,获得了重组载体pTRV2- PDS-VTE1,将该重组载体转化农杆菌GV3101,并侵染番茄.该研究为下一步分析基因沉默后的番茄中维生素E含量的变化提供试验材料；为今后通过转基因技术调节番茄果实的维生素E的合成奠定了基础.
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| 关 键 词： | 番茄 维生素E VIGS 基因沉默 载体构建 |
Construction and Identification VIGS Vector about Vitamin E Biosynthesis Gene of Tomato |
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| LU Liang liang
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FU Da-qi
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LIU Hai-ping
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WANG Xiao-hui
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LUO Yun-bo
,
ZHU Ben-zhong
,
ZUO Jin-hua.Construction and Identification VIGS Vector about Vitamin E Biosynthesis Gene of Tomato[J].Northern Horticulture,2011(16):139-142. |
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| Authors: | LU Liang liang FU Da-qi LIU Hai-ping WANG Xiao-hui LUO Yun-bo ZHU Ben-zhong ZUO Jin-hua |
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| Institution: | LU Liang-liang,FU Da-qi,LIU Hai-ping,WANG Xiao-hui,LUO Yun-bo,ZHU Ben-zhong,ZUO Jin-hua (College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083) |
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| Abstract: | Tomato was used as test material,using RT-PCR to clone part sequence of vitamin E biosynthesis gene's(tocopherol cyclase VTE1) from the tomato fruit.Fragment length was 420 bp.Sequence analysis showed that gene homology of this fragment and VTE1 was 95%.Then XhoI,KpnI restriction sites connected VTE1 with pTRV2-PDS plasmid.The recombinant vector pTRV2-PDS-VTE1 was obtained.And the recombinant vector was transformed into Agrobacterium.The study provided the experimental material for using VIGS technology to ... |
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| Keywords: | tomato vitamin E VIGS gene silencing vector construction |
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