| 低浓度SA诱导桃叶片PGIP基因的表达变化 |
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| 引用本文： | 魏平,张军科.低浓度SA诱导桃叶片PGIP基因的表达变化[J].北方园艺,2011(17):128-130. |
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| 作者姓名： | 魏平 张军科 |
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| 作者单位： | 西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌712100 |
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| 基金项目： | 国家自然科学基金资助项目(30671447) |
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| 摘 要： | 通过改良的CTAB方法提取桃叶片总RNA,根据桃PGIP基因开放阅读框设计特异引物,以荧光实时定量PCR技术分析低浓度SA诱导处理后桃叶片PGIP基因的表达水平变化.结果表明:0.002 mmol/L SA诱导处理桃叶片后引起PGIP基因表达水平上升,在2h出现峰值,表达峰值时(2 h)的表达量是最低点(8 h)表达量的2.4倍.清水对照在0～8 h内PGIP基因的表达几乎没有变化,由此可见,0.002 mmol/L的SA对桃叶片PGIP基因的表达有促进作用.
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| 关 键 词： | 桃 定量PCR PGIP基因 水杨酸 |
The Effect of SA Induction on the Expression of PGIP Gene in Peach Leaves |
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| WEI Ping
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ZHANG Jun-ke.The Effect of SA Induction on the Expression of PGIP Gene in Peach Leaves[J].Northern Horticulture,2011(17):128-130. |
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| Authors: | WEI Ping ZHANG Jun-ke |
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| Institution: | WEI Ping,ZHANG Jun-ke(College of Horticulture,Northwest Agricultural and Forestry University,Key Laboratory of Northwest Horticultural Plant Germplasm Application of Ministry of Agriculture,Yangling,Shaanxi 712100) |
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| Abstract: | In this research,the total RNA of peach leaves were extracted with the improved CTAB method.Specific oligonucleotide primers were designed according to the peach PGIP gene's open reading frame.According to the method of real-time fluorescence quantitative PCR,the expression changing of peach leaves and peel were analyzed.The result showed that 0.002 mmol/L SA treatment resulted in up-regulation of PGIP gene expression.The expression peak occurred 2 h after treatment.The highest level(2 h) was 2.4 times high... |
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| Keywords: | Prunus persica(L ) Batch real-time quantitative PCR PGIP gene salicylic acid |
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