| 牡丹EST序列中的微卫星分析 |
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| 引用本文： | 贾小平,史国安,孔祥生,范丙友,张龙. 牡丹EST序列中的微卫星分析[J]. 北方园艺, 2011, 0(17): 131-133 |
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| 作者姓名： | 贾小平 史国安 孔祥生 范丙友 张龙 |
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| 作者单位： | 河南科技大学农学院,河南洛阳,471003;河南科技大学农学院,河南洛阳,471003;河南科技大学农学院,河南洛阳,471003;河南科技大学农学院,河南洛阳,471003;河南科技大学农学院,河南洛阳,471003 |
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| 基金项目： | 河南科技大学青年科学基金资助项目(2009QN001) |
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| 摘 要： | NCBI的dbEST数据库中2 204条牡丹EST序列经过预处理获得2 048个高质量的序列,经拼接,获得318个Contigs和636个Singlets.其中142条拼接序列共检测出167个微卫星(简单序列重复,SSR),平均1.18个SSR/Contig或Singlet.这些微卫星从二至四核苷酸重复都有出现,二、三核苷酸重复类型占多数(97.61％),其中二核苷酸重复为46.71％,三核苷酸重复为50.90％.二核苷酸重复类型中出现最多的是AG/TC和GA/CT,分别占SSR总数的20.36％和16.77％,三核苷酸重复类型以AGA/TCT、GAT/ATC最多,分别占总SSR的5.40％.最后对含有微卫星的Contigs和Singlets进行基因注释、功能分类.
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| 关 键 词： | 牡丹 EST序列 微卫星分布 功能注释 |
Analysis of Microsatellites in Tree Peony EST Sequences |
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| JIA Xiao-ping , SHI Guo-an , KONG Xiang-sheng , FAN Bing-you , ZHANG Long. Analysis of Microsatellites in Tree Peony EST Sequences[J]. Northern Horticulture, 2011, 0(17): 131-133 |
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| Authors: | JIA Xiao-ping SHI Guo-an KONG Xiang-sheng FAN Bing-you ZHANG Long |
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| Affiliation: | JIA Xiao-ping,SHI Guo-an,KONG Xiang-sheng,FAN Bing-you,ZHANG Long(College of Agriculture,Henan University of Science and Technoloy,Luoyang,Henan 471003) |
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| Abstract: | After precleaning 2 204 tree peony(Paeonia suffruticosa Andrews) expressed sequence tags(ESTs) from dbEST of NCBI,2 048 high-quality ESTs were obtained.318 contigs and 636 singlets were obtained by assembling of these 2 048 ESTs.167 microsatellites(simple sequence repeats,SSRs) were found in 142 of the 954 assembled sequences,on average 1.18 SSRs per assembled sequence.The repeat unit of SSRs included from dinucleotide repeat to tetramer repeat,most of the SSRs were dinucleotide repeat type and trinucleotid... |
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| Keywords: | tree peony EST sequence microsatellite distribution function annotation |
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