| 三苞唇柱苣苔花梗的离体培养 |
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| 引用本文： | 韦啸,唐赛春,黄素梅. 三苞唇柱苣苔花梗的离体培养[J]. 北方园艺, 2011, 0(16): 144-147 |
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| 作者姓名： | 韦啸 唐赛春 黄素梅 |
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| 作者单位： | 广西壮族自治区永福县林业局,广西永福,541800;中国科学院广西植物研究所,广西桂林,541006;中国科学院广西植物研究所,广西桂林541006;广西农业科学院生物技术研究所,广西南宁530007 |
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| 摘 要： | 以三苞唇柱苣苔带腋芽花梗为外植体,对外植体消毒、不定芽的诱导与增殖、生根培养以及生根苗的移栽等组织培养相关技术进行研究.结果表明:外植体的消毒以0.1％的升汞消毒7 min最佳,其成活率为73.3％0；MS基本培养基适合于芽的诱导及生长；不定芽诱导及增殖的最佳培养基为:MS+BA 0.5mg/L+NAA 0.1mg/L；最佳的生根培养基为:MS+IBA0.3 mg/L,最佳的移栽基质为腐殖土,移栽成活率为95％.
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| 关 键 词： | 三苞唇柱苣苔 花梗 离体培养 |
In vitro Culture of Pedicel from Chirita tribracteata W.T.Wang |
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| WEI Xiao , TANG Sai-chun , HUANG Su-mei. In vitro Culture of Pedicel from Chirita tribracteata W.T.Wang[J]. Northern Horticulture, 2011, 0(16): 144-147 |
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| Authors: | WEI Xiao TANG Sai-chun HUANG Su-mei |
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| Affiliation: | WEI Xiao1,TANG Sai-chun2,HUANG Su-mei2,3 (1.Yongfu Forestry Bureau,Guangxi Zhuang Autonomous Region,Yongfu,Guangxi 541800,2.Guangxi Institute of Botany,Chinese Academy of Sciences,Guilin,Guangxi 541006,3.Biotechnology Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007) |
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| Abstract: | Using the pedicels with an axillary bud from Chirita tribracteata W.T.Wang as materials,technologies related to tissue culture(including explant sterilization,induction and proliferation of adventition buds,rooting culture and transplanting of planets) were studied.The results showed that the opitimal sterilization time was about 7 min,and its surivial rate was 73.3%;for induction and proliferation of adventition buds,MS was the optimal basal medium,and the opitimal medium was MS+BA 0.5 mg/L+NAA 0.1 mg/L;th... |
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| Keywords: | Chirita tribracteataW.T.Wang pedicel in vitro culture |
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