| 羽叶薰衣草的组织培养 |
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| 引用本文： | 邵明月,叶子优,陈梦菲,顾福根.羽叶薰衣草的组织培养[J].现代农业科技,2011(22):50-51. |
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| 作者姓名： | 邵明月 叶子优 陈梦菲 顾福根 |
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| 作者单位： | 苏州大学医学部,江苏苏州,215123 |
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| 基金项目： | 苏州大学第十三批大学生课外学术科研基金 |
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| 摘 要： | 以羽叶薰衣草(Lavandula pinnata L.)茎段为外植体进行离体培养,探讨羽叶薰衣草外植体的消毒灭菌、增殖培养、生根培养、炼苗移栽等一系列技术措施。结果表明:用0.1%HgCl2处理羽叶薰衣草外植体6~8 min的效果较好;外植体在含0.8 mg/L 6-BA及0.1 mg/LNAA的MS培养基中培养,增殖效果较好,培养42 d后,平均增殖系数达11.3;较合适的试管苗生根培养基为含0.2 mg/L NAA的1/2 MS培养基,培养21 d后,生根率为98.2%,平均每株生根12.5条;炼苗后移栽成活率达95.0%以上。
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| 关 键 词： | 羽叶薰衣草 组织培养 炼苗 移栽 |
Tissue Culture of Lavandula pinnata L. |
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| Authors: | SHAO Ming-yue YE Zi-you CHEN Meng-fei GU Fu-gen |
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| Institution: | SHAO Ming-yue YE Zi-you CHEN Meng-fei GU Fu-gen (Medicine Department,Suzhou University,Suzhou Jiangsu 215123) |
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| Abstract: | The in vitro culture of Lavandula pinnata L.was established by using the nodal stem segments.The method of sterilization of explants,the effects of different plant growth regulators on the multiplication and rooting,the hardening and transplantation of test-tube plantlets were investigated.The results showed that soaking the explants in 0.1% HgCl2 for 6~8 minutes was a reliable method for obtaining sterile explants.Initial sterile culture was performed on MS medium supplemented with 0.8 mg/L 6-BA and 0.1 mg... |
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| Keywords: | Lavandula pinnata L tissue culture hardening transplantation |
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