| UV法测定北五加皮的不同时间水提物中绿原酸的含量 |
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| 引用本文： | 关颖丽,杨荣艳,谢文新.UV法测定北五加皮的不同时间水提物中绿原酸的含量[J].北方园艺,2011(14):177-178. |
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| 作者姓名： | 关颖丽 杨荣艳 谢文新 |
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| 作者单位： | 通化师范学院制药与食品科学系,吉林,通化,134002;四平市食品药品检验所,吉林,四平,136001 |
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| 基金项目： | 吉林省教育厅科研基金资助项目(吉教科合字[2009]第464号) |
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| 摘 要： | 以绿原酸为对照品,利用UV法在UV 326 nm处进行北五加皮不同时间水提物中绿原酸含量的测定。结果表明:绿原酸在0.0017~0.0101 mg/mL的范围内呈良好线性关系(r=0.9992),平均加样回收率为99.1%(n=6,RSD=1.09%)。确定水提取北五加皮中绿原酸的最佳时间为2 h,该方法简单快速、易于操作,试验结果可靠。
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| 关 键 词： | 北五加皮 绿原酸 UV |
Study on the Content of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times by UV |
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| GUAN Ying-li,YANG Rong-yan,XIE Wen-xin.Study on the Content of the Chlorogenic Acid in Water Extract of Cortex periplocae at the Different Times by UV[J].Northern Horticulture,2011(14):177-178. |
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| Authors: | GUAN Ying-li YANG Rong-yan XIE Wen-xin |
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| Institution: | GUAN Ying-li1,YANG Rong-yan2,XIE Wen-xin1(1.Department of Pharmaceutics and Food Science,Tonghua Teachers College,Tonghua,Jilin 134002,2.Siping Institute of Pharmaceutical and Food,Siping,Jilin 136001) |
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| Abstract: | The content of chlorogenic acid in water extract of Cortex periplocae at the different times were determined by UV.Using chlorogenic acid as a standard substance,the content was determined by spectrophotometer at 326 nm.The linear range of rutin were 0.0017 ~0.0101 mg/mL(r=0.9992).The average recoveries was 99.1%(n=6,RSD=1.09%).Two hours was the best time to extraction of chlorogenic acid.The method was simple,fast,feasible and accurate. |
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| Keywords: | Cortex periplocae chlorogenic acid UV |
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