| Abstract: | Spleen cells from a pig hyperimmunized with Escherichia coli were fused with nonproducer mouse plasmacytoma cells. Stable hybridoma lines secreting porcine immunoglobulins were obtained. One line secreted monoclonal porcine immunoglobulin G, which reacted specifically with E coli in an enzyme-linked immunosorbent assay and precipitated a polypeptide of molecular weight of 50,000. This is the first report of a stable porcine-murine hybridoma producing porcine antibody of defined specificity. |
